A polarographic method for following the rates of cholinesterase-catalyzed hydrolyses of acetylthiocholine
dc.contributor.author | Ridgway, Thomas H. | en_US |
dc.contributor.author | Mark, Jr. , Harry B. | en_US |
dc.date.accessioned | 2006-04-13T14:41:30Z | |
dc.date.available | 2006-04-13T14:41:30Z | |
dc.date.issued | 1965-08 | en_US |
dc.identifier.citation | Ridgway, Thomas H., Mark, Jr., Harry B. (1965/08)."A polarographic method for following the rates of cholinesterase-catalyzed hydrolyses of acetylthiocholine." Analytical Biochemistry 12(2): 357-366. <http://hdl.handle.net/2027.42/31994> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DW3MKR-59/2/81c2798c393187091c9b4d0c2fd2f997 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31994 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14340030&dopt=citation | en_US |
dc.description.abstract | A rapid and simple polarographic method for following the rates of the cholinesterase-catalyzed hydrolysis of acetylthiocholine and the rates of inhibition of this hydrolysis is described. This method has the advantage that it can follow quite rapid reactions, as meaningful data can be obtained after 10 sec of mixing of the solutions. The method follows the rate of increase of the anodic polarographic wave of thiocholine produced by the hydrolysis. The validity of the method was determined by comparing the rates of production of thiocholine spectrophotometrically and polarographically. Also the values of Km for the hydrolysis, and Km and K1 for the inhibition reaction of neostigmine were determined and compared to values reported in the literature. | en_US |
dc.format.extent | 604599 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A polarographic method for following the rates of cholinesterase-catalyzed hydrolyses of acetylthiocholine | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.identifier.pmid | 14340030 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31994/1/0000036.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(65)90103-X | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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