Protein-carbohydrate interaction : III. Agar gel-diffusion studies on the interaction of concanavalin A, a lectin isolated from jack bean, with polysaccharides
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.contributor.author | So, Lucy L. | en_US |
dc.date.accessioned | 2006-04-13T14:41:36Z | |
dc.date.available | 2006-04-13T14:41:36Z | |
dc.date.issued | 1965-08 | en_US |
dc.identifier.citation | Goldstein, I. J., So, Lucy L. (1965/08)."Protein-carbohydrate interaction : III. Agar gel-diffusion studies on the interaction of concanavalin A, a lectin isolated from jack bean, with polysaccharides." Archives of Biochemistry and Biophysics 111(2): 407-414. <http://hdl.handle.net/2027.42/31996> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW2FW3-11H/2/25084d0219bef8a00d60b54abda9bced | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31996 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4955563&dopt=citation | en_US |
dc.description.abstract | Concanavalin A, a protein isolated from the jack bean (Canavalia ensiformis), has previously been shown to form a precipitate with glycogen and yeast mannan, and more recently with dextrans and amylopectin. Double-diffusion precipitation studies in agar gel have been found to afford a very sensitive and satisfactory method for investigating the interaction of concanavalin A with certain types of branched, glucose- and mannose- containing polysaccharides. In this manner concanavalin A can be used as a reagent for the detection and preliminary characterization of various types of polysaccharides. Bacterial levans as well as certain plant fructans also form precipitation bands with concanavalin A. This observation represents the first report of such an interaction. The method may also provide an indication as to the heterogeneity of polysaccharide preparations that react with concanavalin A as well as affording preliminary information on the relative molecular size of a series of reactive polysaccharides. Inhibition of the precipitation bands by low molecular weight carbohydrate molecules may be demonstrated. These inhibition studies reveal the highly specific nature of the combining sites of the concanavalin A molecule. | en_US |
dc.format.extent | 3460221 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Protein-carbohydrate interaction : III. Agar gel-diffusion studies on the interaction of concanavalin A, a lectin isolated from jack bean, with polysaccharides | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA; Department of Biochemistry, School of Medicine, State University of New York at Buffalo, Buffalo, New York, USA. | en_US |
dc.contributor.affiliationum | Department of Biochemistry, School of Medicine, State University of New York at Buffalo, Buffalo, New York, USA; Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA. | en_US |
dc.identifier.pmid | 4955563 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31996/1/0000038.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(65)90203-1 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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