Enrichment of proteins with amino acid residues, employing p, p'-difluoro-m, m'-dinitrodiphenyl sulfone,

Abstract

The bifunctional reagent, p, p'-difluoro-m,m'-dinitrodiphenyl sulfone, was employed to conjugate a variety of amino acids to the protein, gelatin. Among the amino acids tried, tyrosine conjugated most readily. The tyrosyl derivative of gelatin was characterized by electrophoretic, ultracentrifugal, and spectral studies. The conjugation of tyrosine to gelatin appeared to take place through the phenolic groups, although it was also shown to occur through the amino groups when the phenolic groups were blocked.