Inhibition of protein synthesis in cell-free systems by homocitrullylamino adenosine

Abstract

Homocitrullylamino adenosine inhibits the incorporation of [14C]amino acids into a hot acid-insoluble form in cell-free systems derived from Escherichia coli and rat liver. The concentration necessary for one-half maximal inhibition in the E. coli system is 1.1[middle dot]10-4 M. Addition of the intact molecule is necessary for the maximum inhibition. The inhibition does not appear to involve the activation of the amino acids or their transfer to s-RNA, but rather exerts its effect, like puromycin, at the level involving the s-RNA ribosomal complex.