Studies on the estimation of deoxyribonucleic acid and ribonucleic acid in rat brain
dc.contributor.author | Santen, Richard J. | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.date.accessioned | 2006-04-13T14:53:14Z | |
dc.date.available | 2006-04-13T14:53:14Z | |
dc.date.issued | 1963 | en_US |
dc.identifier.citation | Santen, Richard J., Agranoff, Bernard W. (1963)."Studies on the estimation of deoxyribonucleic acid and ribonucleic acid in rat brain." Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis 72(): 251-262. <http://hdl.handle.net/2027.42/32264> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B73G8-486T654-1C/2/3ee223be010f9d1cb850341826857b8a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/32264 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=13976364&dopt=citation | en_US |
dc.description.abstract | A method for the estimation of DNA and RNA in rat brain is described. The principal modifications of preexisting methods involve initial lipid extraction with chloroform-methanol and continuous stirring during alkaline hydrolysis of RNA and acid extraction of DNA. Comparison of ultraviolet-absorptive methods, phosphate determination, and orcinol reaction for RNA, shows a greater degree of correspondence than has previously been reported for brain tissue. Also, a relatively high degre of reproducibility for each of the methods used is obtained with a two-wavelength ultraviolet method. In addition to chemical separation of DNA and RNA components suitable for isotopic studies, an intact lipid fraction is obtained without acid or heating steps.Intracranial injection of [3H]thymidine into young rats resulted in incorporation of as much as 10% of the injected dose into DNA in 2 h.The DNA per cell nucleus obtained from brain homogenates was found to be 6.4[middle dot]10-12 g. On this basis, there 5.5[middle dot]108 cells in the brain of a young adult rat. | en_US |
dc.format.extent | 682398 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Studies on the estimation of deoxyribonucleic acid and ribonucleic acid in rat brain | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.identifier.pmid | 13976364 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/32264/1/0000326.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2787(63)90241-7 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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