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Microdetermination of dihydroxyacetone in biological fluids
dc.contributor.authorTsao, Makepeace U.en_US
dc.contributor.authorSchwartz, E. L.en_US
dc.date.accessioned2006-04-13T14:57:49Z
dc.date.available2006-04-13T14:57:49Z
dc.date.issued1961-04en_US
dc.identifier.citationTsao, M. U., Schwartz, E. L. (1961/04)."Microdetermination of dihydroxyacetone in biological fluids." Analytical Biochemistry 2(2): 107-113. <http://hdl.handle.net/2027.42/32368>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DX4787-4Y/2/45460c6f10f1bd1b2af8e8a8ff263c43en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/32368
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=13778338&dopt=citationen_US
dc.description.abstractDihydroxyacetone is reacted with an excess of 2,4-dinitrophenylhydrazine. The excess reagent is removed by the addition of pyruvic acid and followed by partition between benzene and sodium bicarbonate solution. The benzene layer containing the 2,4-dinitrophenylosazone of dihydroxyacetone is evaporated, and the color developed with the addition of alcoholic sodium hydroxide is measured. Glucose, an interfering material in blood or urine, is removed by an oxidase. Other interference is corrected by the removal of dihydroxyacetone with alkali. Effective range of this method is from 5 [mu]g dihydroxyacetone. The precision of the method has been measured.en_US
dc.format.extent441062 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleMicrodetermination of dihydroxyacetone in biological fluidsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumDepartment of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USAen_US
dc.identifier.pmid13778338en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/32368/1/0000443.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(61)90059-8en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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