The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty acids,
dc.contributor.author | Morell, Pierre | en_US |
dc.contributor.author | Costantino-Ceccarini, Elvira | en_US |
dc.contributor.author | Radin, Norman S. | en_US |
dc.date.accessioned | 2006-04-17T15:05:59Z | |
dc.date.available | 2006-04-17T15:05:59Z | |
dc.date.issued | 1970-12 | en_US |
dc.identifier.citation | Morell, Pierre, Costantino-Ceccarini, Elvira, Radin, Norman S. (1970/12)."The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty acids,." Archives of Biochemistry and Biophysics 141(2): 738-748. <http://hdl.handle.net/2027.42/32654> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW2N34-2J7/2/c61c8818ae5573c4d16f3eb28ff83246 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/32654 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5497154&dopt=citation | en_US |
dc.description.abstract | Incubation of microsomes from mouse brain with UDP-gal, a long-chain base, and 14C-fatty acyl-CoA resulted in formation of NFA-cerebroside labeled in the fatty acid residue. The rate of synthesis was ten times that of the system lacking UDP-gal or long chain base. The incubation system also formed NFA-ceramide, at a much higher rate. Stearoyl- and lignoceroyl-CoA were much more effective in forming ceramide and cerebroside than palmitoyl- and oleoyl-CoA, a correlation which suggests that ceramide is an intermediate in cerebroside biosynthesis.When NFA-ceramide, together with a phospholipid mixture, was incubated with labeled UDP-gal, NFA-cerebroside was formed. Utilization of the exogenous ceramide was demonstrated by a number of Chromatographie procedures. Under the same conditions, but with labeled UDP-glc, the corresponding glucose-containing cerebroside was formed, at a somewhat higher rate.The organic synthesis of labeled lignoceric acid and lignoceroyl -sphingosine is described. Also described is a simplified method for large-scale preparation of purified -sphingosine. The labeled ceramide was utilized by the microsomes for the formation of galactosyl lignoceroyl sphingosine.Under a variety of conditions the microsomes failed to convert psychosine and labeled acyl-CoA to cerebroside. | en_US |
dc.format.extent | 2437136 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty acids, | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Saul R. Korey Department of Neurology and the Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA; The Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Saul R. Korey Department of Neurology and the Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA; The Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Saul R. Korey Department of Neurology and the Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA; The Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 5497154 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/32654/1/0000019.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(70)90192-X | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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