Temperature dependence of amino acid transport in ehrlich ascites cells: with results which bear on the A-L distinction
dc.contributor.author | Jacquez, John A. | en_US |
dc.contributor.author | Sherman, James H. | en_US |
dc.contributor.author | Terris, James | en_US |
dc.date.accessioned | 2006-04-17T15:11:30Z | |
dc.date.available | 2006-04-17T15:11:30Z | |
dc.date.issued | 1970-03-17 | en_US |
dc.identifier.citation | Jacquez, John A., Sherman, James H., Terris, James (1970/03/17)."Temperature dependence of amino acid transport in ehrlich ascites cells: with results which bear on the A-L distinction." Biochimica et Biophysica Acta (BBA) - Biomembranes 203(1): 150-166. <http://hdl.handle.net/2027.42/32781> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1T-47T2TSD-2B/2/b97d800428bb62babfba0b09cf81b638 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/32781 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5445672&dopt=citation | en_US |
dc.description.abstract | We have examined the temperature dependence of maximal flux, JM, the half-saturation value, Km, and of the linear components, k, of the initial transport flux for -methionine, -tryptopah, glycine, -alanine, [alpha]-aminoisobutyric acid and -phenylalanine in Ehrlich ascites cells. For -alanine, [alpha]-aminoisobutyric acid and -phenylalanine we examined the initial flux in transport and in exchange for cells in Na+-containing and in Na+-free media.The maximal flux in transport falls with decrease in temperature, the activation energies falling in the range 11-20 kcal/mole.The Km decreased with decrease in temperature for -phenylalanine and -tryptophan with apparent activation energies of 20 and 15 kcal/mole respectively, For -methionine, glycine and -alanine the Km showed no dependence on temperature whereas for [alpha]-aminoisobutyric acid in increased as the temperature fell. For all amino acids, the Km appeared to be more variable a parameter of the initial flux than was the maximal flux.The results for the linear component of the uptake, k, also were highly variable, but k did decrease with a fall in temperature with activation energies falling in the range 5.8-17.7 kcal/mole. | en_US |
dc.format.extent | 1011530 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Temperature dependence of amino acid transport in ehrlich ascites cells: with results which bear on the A-L distinction | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physiology, The University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationum | Department of Physiology, The University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationum | Department of Physiology, The University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.identifier.pmid | 5445672 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/32781/1/0000154.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2736(70)90045-3 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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