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Fractionation of the insoluble brain proteins with acrylamide electrophoresis

dc.contributor.authorLim, Ramonen_US
dc.contributor.authorTadayyon, Encyen_US
dc.date.accessioned2006-04-17T15:11:35Z
dc.date.available2006-04-17T15:11:35Z
dc.date.issued1970-03en_US
dc.identifier.citationLim, Ramon, Tadayyon, Ency (1970/03)."Fractionation of the insoluble brain proteins with acrylamide electrophoresis." Analytical Biochemistry 34(1): 9-15. <http://hdl.handle.net/2027.42/32783>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DVNM5J-6B/2/297fcfc94a44b6987caadd2cce4aeb16en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/32783
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5440919&dopt=citationen_US
dc.description.abstractA method for fractionating the insoluble proteins of the brain with polyacrylamide electrophoresis is described. The procedure consists of dissolving the membrane proteins in a solution of urea, alkali, mercapto-ethanol, and Triton, and then conducting the separation in gels containing urea and Triton in discontinuous acidic buffers. As many as 30 sharp bands are discernable in the gel out of an insoluble brain fraction. The reproducibility of the band pattern is demonstrable in a gel slab system.en_US
dc.format.extent3100162 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleFractionation of the insoluble brain proteins with acrylamide electrophoresisen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumMental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumMental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.identifier.pmid5440919en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/32783/1/0000156.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(70)90080-1en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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