Purification of a phospholipase C froM Bacillus cereus
dc.contributor.author | Kleiman, Jay H. | en_US |
dc.contributor.author | Lands, William E. M. | en_US |
dc.date.accessioned | 2006-04-17T15:14:52Z | |
dc.date.available | 2006-04-17T15:14:52Z | |
dc.date.issued | 1969-12-17 | en_US |
dc.identifier.citation | Kleiman, Jay H., Lands, William E. M. (1969/12/17)."Purification of a phospholipase C froM Bacillus cereus." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 187(4): 477-485. <http://hdl.handle.net/2027.42/32856> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-47G41MG-7V/2/bc672a4e40032b620e77ede1455db426 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/32856 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4983040&dopt=citation | en_US |
dc.description.abstract | Phospholipase C activity present in the growth medium of Bacillus cereus was purified 20-fold by chromatography on polyethyleneimine-cellulose columns, or by treatment with protamine sulfate and subsequent chromatography on DEAE-cellulose columns. Purified enzyme preparations retained the ability to hydrolyze ethanolamine phosphoglycerides in the absence of choline phosphoglycerides. A typical preparation had a specific activity of about 9 [mu]moles/min per mg toward purified diacyl glycerophosphoryl ethanolamine and a specific activity of about 15-20 [mu]moles/ min per mg toward diacyl glycerophosphorylmonomethylethanolamine and diacyl glycerophosphoryl choline. Monoacyl glycerophosphate was not hydrolyzed under similar conditions. | en_US |
dc.format.extent | 712099 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Purification of a phospholipase C froM Bacillus cereus | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.identifier.pmid | 4983040 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/32856/1/0000232.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(69)90044-7 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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