Hydroxylation of benzphetamine and other drugs by a solubilized form of cytochrome P-450 from liver microsomes: Lipid requirement for drug demethylation
dc.contributor.author | Lu, Anthony Y. H. | en_US |
dc.contributor.author | Strobel, Henry W. | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-17T15:17:33Z | |
dc.date.available | 2006-04-17T15:17:33Z | |
dc.date.issued | 1969-08-15 | en_US |
dc.identifier.citation | Lu, Anthony Y. H., Strobel, Henry W., Coon, Minor J. (1969/08/15)."Hydroxylation of benzphetamine and other drugs by a solubilized form of cytochrome P-450 from liver microsomes: Lipid requirement for drug demethylation." Biochemical and Biophysical Research Communications 36(4): 545-551. <http://hdl.handle.net/2027.42/32916> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4F02W3B-V/2/25d2fe04a8ce69bf96260af3bef4d210 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/32916 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4390187&dopt=citation | en_US |
dc.description.abstract | A solubilized hepatic microsomal enzyme system previously shown to catalyze the [omega]-hydroxylation of fatty acids also catalyzes the hydroxylation of drugs. Benzphetamine, aminopyrine, ethylmorphine, hexobarbital, norcodeine, and -nitroanisole undergo aerobic demethylation in the presence of NADPH and the resolved enzyme system. The required submicrosomal components for benzphetamine demethylation, as determined either by formaldehyde liberation or by NADPH oxidation, are cytochrome P-450, NADPH-cytochrome P-450 reductase, and a heat-stable lipid fraction. Similar requirements were shown for the oxidation of aminopyrine, ethylmorphine, and hexobarbital. Laurate and benzphetamine were found to be mutually inhibitory, as would be expected if a common "methyl hydroxylase" were involved. The solubilized cytochrome P-450 preparation exhibits a difference spectrum in the presence of benzphetamine with a peak at 392 m[mu] and a trough at 427 m[mu] and difference spectra with aniline and hexobarbital typical of those obtained with the microsomal bound form of this hemoprotein. | en_US |
dc.format.extent | 418289 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Hydroxylation of benzphetamine and other drugs by a solubilized form of cytochrome P-450 from liver microsomes: Lipid requirement for drug demethylation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.identifier.pmid | 4390187 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/32916/1/0000296.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(69)90339-8 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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