Defective phage formation by lysogens of integration deficient phage P22 mutants
dc.contributor.author | Smith, Hamilton O. | en_US |
dc.date.accessioned | 2006-04-17T15:31:23Z | |
dc.date.available | 2006-04-17T15:31:23Z | |
dc.date.issued | 1968-02 | en_US |
dc.identifier.citation | Smith, Hamilton O. (1968/02)."Defective phage formation by lysogens of integration deficient phage P22 mutants." Virology 34(2): 203-223. <http://hdl.handle.net/2027.42/33221> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4C5RBPF-83/2/88b255bfa315bdc16f7b051c82276978 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/33221 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4867906&dopt=citation | en_US |
dc.description.abstract | Integration deficient (L) mutants of phage P22 can be complemented with L+ phage to yield L mutant lysogens. Once established the L prophage is stable in the absence of L+ function. Ultraviolet or thermal induction of L lysogens leads to production of defective phage particles and low yields of infectious phage. The defective particles contain a normal amount of DNA but the DNA is mainly bacterial in origin and appears to derive from the pro C region adjacent to one end of the prophage. The defective lysates transduce pro C at high frequency relative to other bacterial markers. The representation of phage genes is strongly polar with markers distal to pro C being rarely present. It appears that L+ function is required not only for efficient integration of the prophage, but for normal recovery of the prophage genome following induction. | en_US |
dc.format.extent | 1879064 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Defective phage formation by lysogens of integration deficient phage P22 mutants | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, Ann Arbor, Michigan, USA; Lawrence D. Buhl Research Center for Human Genetics, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.identifier.pmid | 4867906 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/33221/1/0000611.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(68)90231-6 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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