Transport of amino acids into the estrogen-primed uterus I. General characteristics of the uptake in vitro
dc.contributor.author | Riggs, Thomas R. | en_US |
dc.contributor.author | Pan, Mary W. | en_US |
dc.contributor.author | Feng, Hsien-Wen | en_US |
dc.date.accessioned | 2006-04-17T15:31:34Z | |
dc.date.available | 2006-04-17T15:31:34Z | |
dc.date.issued | 1968-01-03 | en_US |
dc.identifier.citation | Riggs, Thomas R., Pan, Mary W., Feng, Hsien W. (1968/01/03)."Transport of amino acids into the estrogen-primed uterus I. General characteristics of the uptake in vitro." Biochimica et Biophysica Acta (BBA) - Biomembranes 150(1): 92-103. <http://hdl.handle.net/2027.42/33225> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1T-47T8RXV-1C/2/6a7b3d15f6ce8cee3166a896884597d2 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/33225 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5642638&dopt=citation | en_US |
dc.description.abstract | The uptake of free amino acids by the immature rat uterus has been studied in vitro. 1. 1. The uptake of the model amino acid, [alpha]-aminoisobutyric acid, showed properties of active transport. It was saturable; it occurred against a gradient of the free amino acid; it could be decreased by metabolic inhibitors such as cyanide, 2,4-dinitrophenol and lack of O2; and it was temperature sensitive. It was also decreased by the presence of high levels of other amino acids or of K+, and by the absence of extracellular Na+.2. 2. Under appropriate conditions, 1 [mu]g estradiol injected into the rats could increase the uptake in vitro of [alpha]-aminoisobutyric acid, -serine, -alanine, glycine, -proline, -lysine, 1-aminocyclopentanecarboxylic acid, -valine and -norleucine. As short a time as 1 h in vivo was sufficient for this level of the hormone to stimulate uptake of [alpha]-aminoisobutyric acid, 1-aminocyclopentanecarboxylic acid, -alanine and -proline. A 10-6 M level of estradiol added in vitro stimulated [alpha]-aminoisobutyric acid uptake if incubation periods were extended to 6 h.3. 3. Estradiol increased uptake at all [alpha]-aminoisobutyric acid levels tested (0.3-10 mM). Approximation of kinetic constants showed that the hormone increased the [nu]max of [alpha]-aminoisobutyric acid uptake without altering its Km. | en_US |
dc.format.extent | 701207 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Transport of amino acids into the estrogen-primed uterus I. General characteristics of the uptake in vitro | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich., U.S.A. | en_US |
dc.identifier.pmid | 5642638 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/33225/1/0000615.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2736(68)90012-6 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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