Protein-Carbohydrate interaction : VII. Physical and chemical studies on concanavalin A, the hemagglutinin of the jack bean
dc.contributor.author | Agrawal, B. B. L. | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.date.accessioned | 2006-04-17T15:32:12Z | |
dc.date.available | 2006-04-17T15:32:12Z | |
dc.date.issued | 1968 | en_US |
dc.identifier.citation | Agrawal, B. B. L., Goldstein, I. J. (1968)."Protein-Carbohydrate interaction : VII. Physical and chemical studies on concanavalin A, the hemagglutinin of the jack bean." Archives of Biochemistry and Biophysics 124(): 218-229. <http://hdl.handle.net/2027.42/33239> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DXBHBD-M8/2/4118463a0632f3bd6f28efaa21c432c1 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/33239 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5661602&dopt=citation | en_US |
dc.description.abstract | Concanavalin A yielded a monodiperse pattern in the analytical ultracentrifuge in the pH range 2-5. The sedimentation coefficient, so20,w in pH 5 acetate buffer containing 0.1 NaCl (total [Gamma]/2 = 0.20) was 4S. At pH 7 and above, a two-peak schlieren pattern was observed, the faster component (about 7S) probably representing a dimer. On the basis of an s20,w of 3.9S; a D20,w of 5.43 x 10-7 cm2/second, and a iV of 0.73 ml/gm, a molecular weight of 68,000 was calculated (0.10 acetate, NaCl, [Gamma]r/2 = 0.45) for the homogeneous component at pH 5. From free-boundary electrophoresis data the isoelectric point of Concanavalin A was determined to be 7.1 +/- 0.1. Starch gel electrophoresis patterns of concanavalin A in borate buffer (pH 8.6) were characterized by an anodically migrating streak. Incorporation of -glucose into the gel gave a single sharp band which migrated slowly toward the cathode, the glucose probably inhibiting protein-starch interaction. Gel filtration experiments with Biogel P-100 suggested that at pH 5 the molecular weight of Concanavalin A is in the vicinity of 50,000; at pH 7.5 concanavalin A is completely excluded, an indication that a high molecular weight species probably is formed by the association of subunits. Concanavalin A has an extinction coefficient, E1%1cm of 11.4 +/- 0.1, the molar ratio Mtyrosine/ Mtryptophan being 1.78. Unlike other phytohemagglutinins, concanavalin A does not contain cysteine (or cystine) or carbohydrate. Aspartic acid and serine constitute the most abundant residues, and the protein has a high amide content (68.6 groups/105 gm protein). Amino terminal studies showed the presence of 1.5 moles DNP-alanine/ 68,000 gm protein together with very small amounts of DNP-serine and DNP-glycine. The manganese content of concanavalin A was found to be 0.029 and 0.036%, by activation analysis and emission spectroscopy, respectively. | en_US |
dc.format.extent | 2295411 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Protein-Carbohydrate interaction : VII. Physical and chemical studies on concanavalin A, the hemagglutinin of the jack bean | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.identifier.pmid | 5661602 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/33239/1/0000630.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(68)90322-6 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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