Interactions of substrate and non-substrate effectors with p-hydroxybenzoate hydroxylase from
dc.contributor.author | Spector, T. | en_US |
dc.contributor.author | Massey, Vincent | en_US |
dc.date.accessioned | 2006-04-17T16:20:02Z | |
dc.date.available | 2006-04-17T16:20:02Z | |
dc.date.issued | 1971-12-03 | en_US |
dc.identifier.citation | Spector, T., Massey, V. (1971/12/03)."Interactions of substrate and non-substrate effectors with p-hydroxybenzoate hydroxylase from." Biochemical and Biophysical Research Communications 45(5): 1219-1226. <http://hdl.handle.net/2027.42/33509> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DXKBG4-8C/2/831aaf87d182beb68c9a29bbebc92e48 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/33509 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4400084&dopt=citation | en_US |
dc.description.abstract | 3,4-Dihydroxybenzoate (3,4-DOHB), 2,4-dihydroxybenzoate (2,4-DOHB), and benzoate facilitate the interaction of p-hydroxybenzoate hydroxylase with TPNH. The two dihydroxybenzoate effectors form 1:1 complexes with the enzyme, inducing large spectral perturbations and fluorescence quenching. The dissociation constants for 2,4-DOHB and 3,4-DOHB are 0.15 and 0.50 mM respectively. During the reaction of enzyme with TPNH and oxygen, all the 2,4-DOHB, <5% of the benzoate, and none of the 3,4-DOHB is hydroxylated. | en_US |
dc.format.extent | 442291 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Interactions of substrate and non-substrate effectors with p-hydroxybenzoate hydroxylase from | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, 48104, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, 48104, USA | en_US |
dc.identifier.pmid | 4400084 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/33509/1/0000006.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(71)90148-3 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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