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The preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawing

dc.contributor.authorKnorpp, Charles T.en_US
dc.contributor.authorStarkweather, William H.en_US
dc.contributor.authorSpencer, H. H. (Herbert H.)en_US
dc.contributor.authorWeatherbee, Leeen_US
dc.date.accessioned2006-04-17T16:20:21Z
dc.date.available2006-04-17T16:20:21Z
dc.date.issued1971-12en_US
dc.identifier.citationKnorpp, C. T., Starkweather, W. H., Spencer, H. H., Weatherbee, L. (1971/12)."The preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawing." Cryobiology 8(6): 511-516. <http://hdl.handle.net/2027.42/33516>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WD5-4F1HR49-JP/2/718da93a87965c43cb38a6f9cf50c6c3en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/33516
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5137283&dopt=citationen_US
dc.description.abstractPacked human erythrocytes were frozen in liquid nitrogen using hydroxyethyl starch as a cryoprotective agent. Upon thawing, the cells were washed with a balanced salt solution. The washed cells were stable when resuspended in 0.154 n NaCl. The postthaw saline stability approximated the total recovery after washing the cells. Intracellular potassium loss was 15% immediately postthaw, and an additional 15% loss occurred after washing. These results indicate that HES can be removed from frozen-thawed cells by washing, and that freezing erythrocytes protected by HES does not result in significant losses of intracellular potassium.en_US
dc.format.extent1926166 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleThe preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawingen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelStatistics and Numeric Dataen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMathematicsen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumU.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A.en_US
dc.contributor.affiliationumU.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A.en_US
dc.contributor.affiliationumU.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A.en_US
dc.contributor.affiliationumU.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A.en_US
dc.identifier.pmid5137283en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/33516/1/0000014.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0011-2240(71)90001-0en_US
dc.identifier.sourceCryobiologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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