The preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawing
dc.contributor.author | Knorpp, Charles T. | en_US |
dc.contributor.author | Starkweather, William H. | en_US |
dc.contributor.author | Spencer, H. H. (Herbert H.) | en_US |
dc.contributor.author | Weatherbee, Lee | en_US |
dc.date.accessioned | 2006-04-17T16:20:21Z | |
dc.date.available | 2006-04-17T16:20:21Z | |
dc.date.issued | 1971-12 | en_US |
dc.identifier.citation | Knorpp, C. T., Starkweather, W. H., Spencer, H. H., Weatherbee, L. (1971/12)."The preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawing." Cryobiology 8(6): 511-516. <http://hdl.handle.net/2027.42/33516> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WD5-4F1HR49-JP/2/718da93a87965c43cb38a6f9cf50c6c3 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/33516 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5137283&dopt=citation | en_US |
dc.description.abstract | Packed human erythrocytes were frozen in liquid nitrogen using hydroxyethyl starch as a cryoprotective agent. Upon thawing, the cells were washed with a balanced salt solution. The washed cells were stable when resuspended in 0.154 n NaCl. The postthaw saline stability approximated the total recovery after washing the cells. Intracellular potassium loss was 15% immediately postthaw, and an additional 15% loss occurred after washing. These results indicate that HES can be removed from frozen-thawed cells by washing, and that freezing erythrocytes protected by HES does not result in significant losses of intracellular potassium. | en_US |
dc.format.extent | 1926166 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawing | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | U.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A. | en_US |
dc.contributor.affiliationum | U.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A. | en_US |
dc.contributor.affiliationum | U.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A. | en_US |
dc.contributor.affiliationum | U.S. Veterans Administration Hospital, Medical Research and Departments of Medicine, and Pathology, University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A. | en_US |
dc.identifier.pmid | 5137283 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/33516/1/0000014.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0011-2240(71)90001-0 | en_US |
dc.identifier.source | Cryobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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