Fatty acid and hydrocarbon hydroxylation in yeast: Role of cytochrome P-450 in
dc.contributor.author | Lebeault, Jean-Michel | en_US |
dc.contributor.author | Lode, Eglis T. | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-17T16:29:31Z | |
dc.date.available | 2006-04-17T16:29:31Z | |
dc.date.issued | 1971-02-05 | en_US |
dc.identifier.citation | Lebeault, Jean-Michel, Lode, Eglis T., Coon, Minor J. (1971/02/05)."Fatty acid and hydrocarbon hydroxylation in yeast: Role of cytochrome P-450 in." Biochemical and Biophysical Research Communications 42(3): 413-419. <http://hdl.handle.net/2027.42/33708> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DXKBG4-5G/2/ba51233831453d0b858e131fd72708e9 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/33708 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5542889&dopt=citation | en_US |
dc.description.abstract | A soluble enzyme system which catalyzes the conversion of fatty acids to their [omega]-hydroxy derivatives and -alkanes to primary alcohols has been obtained from a strain of grown on tetradecane as the carbon source. The hydroxylation reaction requires TPNH and molecular oxygen and is inhibited by carbon monoxide. Lauric acid appeared to be the most active of a series of substrates tested. Fractionation of a cell-free extract of the yeast with ammonium sulfate indicated that at least two components are required for maximal activity. One fraction contained cytochrome P-450 as judged by the carbon monoxide difference spectrum of the dithionite-reduced preparation. The hydroxylation activity and the content of both cytochrome P-450 and TPNH-cytochrome reductase (which may function as a cytochrome P-450 reductase) were greatly enhanced in cells grown on hydrocarbon. | en_US |
dc.format.extent | 391753 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Fatty acid and hydrocarbon hydroxylation in yeast: Role of cytochrome P-450 in | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan, USA | en_US |
dc.identifier.pmid | 5542889 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/33708/1/0000220.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(71)90386-X | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.