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Micro method for determination of borohydride with NAD+
dc.contributor.authorWerner, D. A.en_US
dc.contributor.authorHuang, Chin C.en_US
dc.contributor.authorAminoff, Daviden_US
dc.date.accessioned2006-04-17T16:37:22Z
dc.date.available2006-04-17T16:37:22Z
dc.date.issued1973-08en_US
dc.identifier.citationWerner, D. A., Huang, Chin C., Aminoff, David (1973/08)."Micro method for determination of borohydride with NAD+." Analytical Biochemistry 54(2): 554-560. <http://hdl.handle.net/2027.42/33839>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DYM9Y1-7H/2/1a12aca57f447ea30122a7bcc6c8ebc5en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/33839
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4353368&dopt=citationen_US
dc.description.abstractA spectrophotometric method for the determination of borohydride is described. It involves the reduction of NAD+ to a number of isomeric forms of NADH by borohydride. In the standard assay procedure herewith presented, there is a direct proportionality between the absorption at 340 nm and the amount of borohydride in solution over the range 10-100 nmoles, with an effective "molar extinction coefficient" of 12.2 x 103. The method is simple, rapid, and sensitive.en_US
dc.format.extent324823 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleMicro method for determination of borohydride with NAD+en_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104, USA; Department of Internal Medicine (Simpson Memorial Institute), University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104, USA; Department of Internal Medicine (Simpson Memorial Institute), University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104, USA; Department of Internal Medicine (Simpson Memorial Institute), University of Michigan, Ann Arbor, Michigan 48104, USAen_US
dc.identifier.pmid4353368en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/33839/1/0000097.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(73)90387-4en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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