Show simple item record

Synthesis of soluble neuronal proteins in vivo. Age-dependent differences in the incorporation of leucine and phenylalanine

dc.contributor.authorJohnson, D. E.en_US
dc.contributor.authorSellinger, Otto Z.en_US
dc.date.accessioned2006-04-17T16:39:15Z
dc.date.available2006-04-17T16:39:15Z
dc.date.issued1973-05-17en_US
dc.identifier.citationJohnson, D. E., Sellinger, O. Z. (1973/05/17)."Synthesis of soluble neuronal proteins in vivo. Age-dependent differences in the incorporation of leucine and phenylalanine." Brain Research 54(): 129-142. <http://hdl.handle.net/2027.42/33880>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6SYR-4835W1P-C/2/a6da24b96828190aa72f12d470b8355ben_US
dc.identifier.urihttps://hdl.handle.net/2027.42/33880
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4709140&dopt=citationen_US
dc.description.abstract[3H]Leucine and [3H]phenylalamine were injected intracerebrally into 8- and 18-day-old rats and, in addition, [3H]proline was injected into 8-day-old rats only. Neuronal perikarya were isolated from the cerebral cortex 15 min later and the rapidly labeled proteins recovered in the high-speed soluble supernatant fraction were analyzed by polyacrylamide disc gel electrophoresis.Using sodium dodecyl sulfate (SDS)-urea gels, significant differences were noted between the [3H]leucine and the [3H]phenylalanine-labeled proteins of the 8-day-old neuron, the former consisting largely of slow-moving, high molecular weight species, while the latter consisted of fast-moving, small molecular weight species. The pattern of the [3H]proline-labeled proteins conformed generally to that of [3H]-phenylalamine.The trend seen in the 8-day-old neurons was largely reserved in the 18-day-old neurons, demonstrating that the protein synthetic machinery of the cortical neuron undergoes qualitative, precursor-dependent adjustments during its development.Under the conditions of our experiments, no labeling of microtubule proteins was detected. Furthermore, no radioactivity migrating as microtubule protein was found in the vinblastine precipitate of the neuronal soluble proteins.Electrophoresis of a hypotonic extract of the neuronal soluble proteins revealed a number of additional radioactive proteins, presumed to be nascent polypeptides caught in the lumen of the cisternae of the endoplasmic reticulum, while en route to extraperikaryal destinations.en_US
dc.format.extent903829 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleSynthesis of soluble neuronal proteins in vivo. Age-dependent differences in the incorporation of leucine and phenylalanineen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelNeurosciencesen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumLaboratory of Neurochemistry, Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A.en_US
dc.contributor.affiliationumLaboratory of Neurochemistry, Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Mich. 48104, U.S.A.en_US
dc.identifier.pmid4709140en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/33880/1/0000144.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-8993(73)90039-5en_US
dc.identifier.sourceBrain Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.