Evidence for the utilization of host tRNA(m5 U)methylase to modify tRNA coded by phage T4
dc.contributor.author | Bjork, Glenn R. | en_US |
dc.contributor.author | Neidhardt, Frederick C. | en_US |
dc.date.accessioned | 2006-04-17T16:40:40Z | |
dc.date.available | 2006-04-17T16:40:40Z | |
dc.date.issued | 1973-04 | en_US |
dc.identifier.citation | Bjork, Glenn R., Neidhardt, Frederick C. (1973/04)."Evidence for the utilization of host tRNA(m5 U)methylase to modify tRNA coded by phage T4." Virology 52(2): 507-519. <http://hdl.handle.net/2027.42/33911> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4BNVKDB-150/2/25deac78cae0f02deed298c7d4be47a2 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/33911 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4574517&dopt=citation | en_US |
dc.description.abstract | Mutants of Escherichia coli defective only in the biosynthesis of 5-methyluridine (ribothymidine) in their transfer ribonucleic acid (tRNA) were employed to investigate whether phage T4 induces its own tRNA (m5U)methylase and whether T4-specific tRNA contains 5-methyluridine. The amounts of other methylated derivatives in the T4-specific tRNA were also determined. The results establish that T4-specific tRNA contains 5-methyluridine, and that there is an absolute requirement for a functional host tRNA(m5U)methylase to undertake this modification. Comparison of several physical properties of the host enzyme before and after phage T4 infection did not suggest any phage-directed alteration of the enzyme. The distribution of the methylated constituents in T4-specific tRNA is distinguishable from that in host tRNA. This change, however, may simply reflect a different population of tRNA chains produced by the T4 phage, rather than some change in the tRNA methylase activity of the host. | en_US |
dc.format.extent | 1369141 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Evidence for the utilization of host tRNA(m5 U)methylase to modify tRNA coded by phage T4 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 4574517 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/33911/1/0000176.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(73)90346-2 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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