Extractability of lysozyme from bovine nasal cartilage
dc.contributor.author | Sorgente, Nino | en_US |
dc.contributor.author | Hascall, Vincent C. | en_US |
dc.contributor.author | Kuettner, Klaus E. | en_US |
dc.date.accessioned | 2006-04-17T16:45:34Z | |
dc.date.available | 2006-04-17T16:45:34Z | |
dc.date.issued | 1972-10-12 | en_US |
dc.identifier.citation | Sorgente, Nino, Hascall, Vincent C., Kuettner, Klaus E. (1972/10/12)."Extractability of lysozyme from bovine nasal cartilage." Biochimica et Biophysica Acta (BBA) - Enzymology 284(2): 441-450. <http://hdl.handle.net/2027.42/34018> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B73GH-47GH67K-2W/2/330be4664ff0032be7eeaeaa881de4b5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34018 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4564457&dopt=citation | en_US |
dc.description.abstract | Lysozyme (mucopeptide N-acetylmuramylhydrolase, EC 3.2.1.17) is present in the extracellular matrix of bovine nasal cartilage at a concentration of about 0.03 to 0.08 mg per g wet tissue (3-8 mg/100 ml per g). It is extracted from the tissue in guanidinium chloride solutions between 0.3 and 0.8 M. These concentrations are much lower than those which effectively extract most of the proteoglycans from the tissue, 2.5-3.0 M. Lysozyme migrates to the top of CsCl density gradients which are used to purify aggregated or monomer proteoglycan preparations; this suggests that lysozyme is not involved in the aggregation of proteoglycans in vitro. Chondroitinase from Proteus vulgaris effectively removes chondroitin sulfate from cartilage slices without solubilizing lysozyme which indicates that this highly anionic polysaccharide does not immobilize lysozyme in the matrix. Trypsin (EC 3.4.4.4) releases most of the chondroitin sulfate as well as 75% of the lysozyme from the matrix. | en_US |
dc.format.extent | 595943 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Extractability of lysozyme from bovine nasal cartilage | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, Department of Oral Biology, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationother | Rush-Presbyterian-St. Luke's Medical Center, Department of Orthopaedic Surgery, Chicago, Ill. 60612, U.S.A. | en_US |
dc.contributor.affiliationother | Rush-Presbyterian-St. Luke's Medical Center, Department of Orthopaedic Surgery, Chicago, Ill. 60612, U.S.A. | en_US |
dc.identifier.pmid | 4564457 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34018/1/0000294.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2744(72)90141-6 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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