Epimerization of trans-4-hydroxy--proline to cis-4-hydroxy--proline during acid hydrolysis of collagen
dc.contributor.author | Dziewiatkowski, Dominic D. | en_US |
dc.contributor.author | Hascall, Vincent C. | en_US |
dc.contributor.author | Riolo, Rick L. | en_US |
dc.date.accessioned | 2006-04-17T16:45:59Z | |
dc.date.available | 2006-04-17T16:45:59Z | |
dc.date.issued | 1972-10 | en_US |
dc.identifier.citation | Dziewiatkowski, Dominic D., Hascall, Vincent C., Riolo, Rick L. (1972/10)."Epimerization of trans-4-hydroxy--proline to cis-4-hydroxy--proline during acid hydrolysis of collagen." Analytical Biochemistry 49(2): 550-558. <http://hdl.handle.net/2027.42/34027> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DYVHVH-DY/2/f74999167661093bf10b87176fc86ac1 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34027 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5082949&dopt=citation | en_US |
dc.description.abstract | 1. 1. trans-4-Hydroxy--proline is epimerized to cis-4-hydroxy--proline under the conditions generally used for the hydrolysis of proteins for analyses of amino acids.2. 2. The cis epimer elutes from a column of UR-30 resin on a Beckman amino acid analyzer at the same time as threonine and hence would be undetected.3. 3. About 8% of the trans-4-hydroxy--proline in collagen can be epimerized to cis-4-hydroxy--proline in the hydrolysis of collagen with 6 N HCl at 110[deg] for 72 hr.4. 4. A procedure is described whereby the primary amino acids in hydrolyzates are deaminated with nitrous acid and removed so that the amounts of trans-4-hydroxyproline, cis-4-hydroxyproline, and proline can be determined without changing the temperature of the column of UR-30 resin on the Beckman amino acid analyzer. | en_US |
dc.format.extent | 447393 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Epimerization of trans-4-hydroxy--proline to cis-4-hydroxy--proline during acid hydrolysis of collagen | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Dental Research Institute, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Dental Research Institute, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Dental Research Institute, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 5082949 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34027/1/0000304.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(72)90461-7 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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