Effect of a diabetogeic factor from bovine adenohypophysis on dog serum nefa levels

Abstract

1. 1. Fasted dog serum non-esterified fatty acid (NEFA) levels were elevated 10 and 34 hours after administration of a diabetogenic protein (BDF) isolated from bovine adenohypophysis.2. 2. The fasting serum NEFA were elevated in spite of the presence of normal or high glucose and insulin levels.3. 3. After oral glucose administration to BDF-treated animals, a fall of serum NEFA paralleled inversely the increasing levels of blood glucose and serum insulin.4. 4. These data are discussed with respect to the insulin-antagonistic action of BDF. Even though the plasma NEFA fell in response to glucose in BDF-treated animals, the NEFA during the entire 3-hour glucose-tolerance test were always significantly higher than the levels observed in the control tests.5. 5. Since BDF is similar to the diabetogenic protein isolated from the urine of patients with lipoatrophic diabetes, its lipid-mobilizing action might help to explain the lipoatrophy, hyperlipaemia, diabetes mellitus, and insulin resistance seen in lipoatrophic diabetic patients.