Computer program sequence for analysis and summary of radioimmunoassay data

Abstract

A sequence of three programs is described for efficient design and analysis of radio-immunoassays. The first program designs the assay; the second program analyzes all available data according to the organizational base provided by the first program; and the third program summarizes the results of several assays in either tabular or graphical form. The analysis program uses a logit response-log dose transformation to obtain a linear inhibition curve for all preparations assayed at multiple levels. The curves are subjected to a weithted, least squares, regression analysis. All curves are tested for linearity and parallelism with the curve chosen as standard, and a weighted mean potency estimate with error limits is computed for all preparations run at single or multiple dose levels with any degree of replication.