Thyroid hormone action on mitochondria : III. Resolution of iodine-containing mitochondria and subfractions by zonal centrifugation
dc.contributor.author | Dimino, Michael J. | en_US |
dc.contributor.author | Kuras, Rosemary A. | en_US |
dc.contributor.author | McCleary, Alan R. | en_US |
dc.contributor.author | Hoch, Frederic L. | en_US |
dc.date.accessioned | 2006-04-17T16:48:55Z | |
dc.date.available | 2006-04-17T16:48:55Z | |
dc.date.issued | 1972-06 | en_US |
dc.identifier.citation | Dimino, Michael J., Kuras, Rosemary A., McCleary, Alan R., Hoch, Frederic L. (1972/06)."Thyroid hormone action on mitochondria : III. Resolution of iodine-containing mitochondria and subfractions by zonal centrifugation." Archives of Biochemistry and Biophysics 150(2): 618-623. <http://hdl.handle.net/2027.42/34090> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW3C09-WP/2/1f7a9001f93952157139235a6a13c4bd | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34090 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5044043&dopt=citation | en_US |
dc.description.abstract | Mitochondria from the livers of normal rats and -thyroxine (LT4)-injected rats (5 [mu]g/g, 2 hr before killing) were studied by rate zonal centrifugation to detect early changes in physical properties and in composition. LT4 treatment causes the fractions containing intact liver mitochondria (as judged by succinoxidase activity) to significantly shift their distribution to the heavier end of a linear sucrose gradient, indicating an increase in sedimentation rate. Iodine distribution follows this shift in mitochondria. Iodine concentration increases 26-fold in mitochondria from LT4-treated rats. In addition, iodine concentrates in the less dense part of the gradient in the LT4-treated group. Submitochondrial particles from the livers of injected rats were prepared by 15 min sonication and resolved by isopycnic zonal centrifugation. Distributions of iodine, cytochrome aa3, RNA and monoamine oxidase activity indicate that LT4 is associated with the inner membrane of mitochondria. | en_US |
dc.format.extent | 520720 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Thyroid hormone action on mitochondria : III. Resolution of iodine-containing mitochondria and subfractions by zonal centrifugation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 5044043 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34090/1/0000372.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(72)90081-1 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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