Morphogenic effects of halogenated thymidine analogs on drosophila II. Incorporation of 5-bromodeoxyuridine into DNA

Abstract

When 5-bromodeoxyuridine is fed to Drosophila larvae, a variety of morphogenic lesions are found in the hatching adults. Experiments were undertaken to determine whether 5-bromodeoxyuridine is incorporated into Drosophila DNA under the conditions which induce developmental modifications.Radioautographic techniques were used to examine the distribution of [3H]-bromodeoxyuridine in Drosophila larvae. Label was found in nuclei of the larval tissues and the imaginal disc cells as well as the gonads.DNA was isolated from Drosophila larvae which were fed 5-bromodeoxyuridine alone or in the presence of a thymidylate inhibitor, and these samples were examined by CsCl equilibrium density-gradient centrifugation. In the presence of 5-fluorouracil the amount of 5-bromodeoxyuridine incorporated into Drosophila DNA was increased, and this difference was reflected as a relative increase in the denser component of BUdR-DNA in CsCl density gradients.