Quantitative study of the effects of denervation and castration on the levator ani muscle of the rat
dc.contributor.author | Nnodim, Joseph O. | en_US |
dc.date.accessioned | 2006-04-19T13:26:39Z | |
dc.date.available | 2006-04-19T13:26:39Z | |
dc.date.issued | 1999-07-01 | en_US |
dc.identifier.citation | Nnodim, Joseph O. (1999)."Quantitative study of the effects of denervation and castration on the levator ani muscle of the rat." The Anatomical Record 255(3): 324-333. <http://hdl.handle.net/2027.42/34285> | en_US |
dc.identifier.issn | 0003-276X | en_US |
dc.identifier.issn | 1097-0185 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34285 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10411399&dopt=citation | en_US |
dc.description.abstract | The levator ani muscle (LA) of the rat is highly androgen-sensitive and, like all skeletal muscles, deteriorates structurally and functionally when denervated. In order to elucidate the interplay of neural and endocrine influences, the separate and combined effects of denervation and castration on myofiber cross-sectional area and nuclear populations were quantitatively studied. In one group of 4-month-old male rats (A), the LA was denervated. Another group (B) was surgically castrated and a third group (C) was both denervated and castrated. The control rats (D) remained both gonad- and nerve-intact. After two months, the LA was obtained for myofiber and nuclear enumeration, cross-sectional area and satellite cell frequency determination. In the denervated muscle of gonad-intact rats (Group A), myofiber cross-sectional area was markedly diminished (265.84 ± 11.38μm 2 ; compared with controls [Group D]: 1519.98 ± 79.41μm 2 ; P < 0.05). Satellite cell nuclei, as a percentage of total sublaminar nuclei (i.e., satellite cell ratio), increased significantly (4.26%, from a control value of 1.91%). Castration alone (Group B) resulted in pronounced myofiber atrophy (mean cross-sectional area: 754.03 ± 89.63μm 2 ) but had no significant effect on satellite cell ratio (2.36%). The combination of castration and denervation (Group C) elicited the same degree of myofiber atrophy as denervation alone (Group A) but had no significant impact on satellite cell ratio. Instead, the nuclear count per myofiber declined to about a third of the control level (300.5 ± 38.49 compared with 861.7 ± 24.8; P < 0.05). The results indicate that the atrophic effects of denervation and castration on the LA are non-synergistic and mechanistically similar. They also show that the inability of satellite cells to respond mitotically to the withdrawal of neural input under disandrogenized conditions is a factor in the myonuclear depletion of the denervated muscle of castrated rats. Anat Rec 255:324–333, 1999. © 1999 Wiley-Liss, Inc. | en_US |
dc.format.extent | 383488 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Quantitative study of the effects of denervation and castration on the levator ani muscle of the rat | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, Michigan, 48109 ; Department of Anatomy and Cell Biology, University of Michigan Medical School, 1150 Medical Center Drive, Ann Arbor, MI 48109. Fax: 734-763-1166. | en_US |
dc.identifier.pmid | 10411399 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34285/1/8_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-0185(19990701)255:3<324::AID-AR8>3.0.CO;2-1 | en_US |
dc.identifier.source | The Anatomical Record | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.