Prognostic factors in oral cavity and oropharyngeal squamous cell carcinoma
Marcus, Benjamin; Arenberg, Douglas A.; Lee, Julia S.; Kleer, Celina G.; Chepeha, Douglas B.; Schmalbach, Cecelia E.; Islam, Mozaffarul; Paul, Supriti; Pan, Quintin; Hanash, Samir M.; Kuick, Rork; Merajver, Sofia D.; Teknos, Theodoros N.
2004-12-15
Citation
Marcus, Benjamin; Arenberg, Douglas; Lee, Julia; Kleer, Celina; Chepeha, Douglas B.; Schmalbach, Cecelia E.; Islam, Mozaffarul; Paul, Supriti; Pan, Quintin; Hanash, Samir; Kuick, Rork; Merajver, Sofia D.; Teknos, Theodoros N. (2004)."Prognostic factors in oral cavity and oropharyngeal squamous cell carcinoma." Cancer 101(12): 2779-2787. <http://hdl.handle.net/2027.42/34388>
Abstract
BACKGROUND The survival of patients with head and neck squamous cell carcinoma (HNSCC) remains unaffected despite recent therapeutic advances. To reverse this trend, reliable and clinically applicable markers of tumor aggressiveness must be identified. One such marker may be the tumor-associated macrophage content. The authors hypothesized that tumor-associated macrophages contribute to HNSCC aggressiveness, and the objective of the current study was to prove this hypothesis using mRNA expression analysis and a large cohort of clinical specimens. METHODS Oligonucleotide microarray analysis was performed on a prospective cohort of 20 patients with previously untreated oral cavity or oropharynx squamous cell carcinoma (OC/OP SCCA) and on normal oropharyngeal mucosa from 4 patients. After determining whether macrophage chemoattractants were produced by tumors, conditioned media from three HNSCC cell lines were used to quantify macrophage migration in an in vitro assay. A high-density tissue microarray of 102 patients with previously untreated OC/OP SCCA was stained immunohistochemically for CD68 to identify tissue macrophages, and the results were correlated with clinicopathologic data and survival. RESULTS Monocyte chemoattractant protein 1 was up-regulated significantly in tumors compared with normal mucosa ( P = 0.0025; fold change = 1.89). All University of Michigan SCC tumor cell line conditioned media caused a significant increase in macrophage migration ( P < 0.05). Tissue microarray data revealed that macrophage content of the primary tumor was associated strongly with lymph node metastasis ( P < 0.0001), extracapsular lymph node spread ( P = 0.0001), and advanced clinical disease stage ( P = 0.0002). When it was evaluated along with other clinicopathologic data, the macrophage content was found to be an independent predictor of lymph node metastasis ( P < 0.0001). CONCLUSIONS Primary tumor macrophage content is a strong predictor of tumor aggressiveness in HNSCC. Cancer 2004. © 2004 American Cancer Society.Publisher
Wiley Subscription Services, Inc., A Wiley Company
ISSN
0008-543X 1097-0142
Other DOIs
PMID
15546137
Types
Article
URI
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15546137&dopt=citationMetadata
Show full item recordRemediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.