Growth of continuous bonelike mineral within porous poly(lactide- co -glycolide) scaffolds in vitro
dc.contributor.author | Murphy, William L. | en_US |
dc.contributor.author | Kohn, David H. | en_US |
dc.contributor.author | Mooney, David J. | en_US |
dc.date.accessioned | 2006-04-19T13:33:22Z | |
dc.date.available | 2006-04-19T13:33:22Z | |
dc.date.issued | 2000-04 | en_US |
dc.identifier.citation | Murphy, William L.; Kohn, David H.; Mooney, David J. (2000)."Growth of continuous bonelike mineral within porous poly(lactide- co -glycolide) scaffolds in vitro ." Journal of Biomedical Materials Research 50(1): 50-58. <http://hdl.handle.net/2027.42/34417> | en_US |
dc.identifier.issn | 0021-9304 | en_US |
dc.identifier.issn | 1097-4636 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34417 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10644963&dopt=citation | en_US |
dc.description.abstract | Strategies to engineer bone have focused on the use of natural or synthetic degradable materials as scaffolds for cell transplantation or as substrates to guide bone regeneration. The basic requirements of the scaffold material are biocompatibility, degradability, mechanical integrity, and osteoconductivity. A major design problem is satisfying each of these requirements with a single scaffold material. This study addresses this problem by describing an approach to combine the biocompatibility and degradability of a polymer scaffold with the osteoconductivity and mechanical reinforcement of a bonelike mineral film. We report the nucleation and growth of a continuous carbonated apatite mineral on the interior pore surfaces of a porous, degradable polymer scaffold via a one step, room temperature incubation process. A 3-dimensional, porous scaffold of the copolymer 85:15 poly(lactide- co -glycolide) was fabricated by a solvent casting, particulate leaching process. Fourier transform IR spectroscopy and scanning electron microscopy (SEM) analysis after different incubation times in a simulated body fluid (SBF) demonstrate the growth of a continuous bonelike apatite layer within the pores of the polymer scaffold. Quantification of phosphate on the scaffold displays the growth and development of the mineral film over time with an incorporation of 0.43 mg of phosphate (equivalent to 0.76 mg of hydroxyapatite) per scaffold after 14 days in SBF. The compressive moduli of polymer scaffolds increased fivefold with formation of a mineral film after a 16-day incubation time as compared to control scaffolds. In summary, this biomimetic treatment provides a simple, one step, room temperature method for surface functionalization and subsequent mineral nucleation and growth on biodegradable polymer scaffolds for tissue engineering. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 50, 50–58, 2000. | en_US |
dc.format.extent | 649517 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Growth of continuous bonelike mineral within porous poly(lactide- co -glycolide) scaffolds in vitro | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109 ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 10644963 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34417/1/8_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-4636(200004)50:1<50::AID-JBM8>3.0.CO;2-F | en_US |
dc.identifier.source | Journal of Biomedical Materials Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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