Nociceptin/orphanin FQ and opioid receptor-like receptor mRNA expression in dopamine systems
dc.contributor.author | Norton, Camille S. | en_US |
dc.contributor.author | Neal, Charles Richard | en_US |
dc.contributor.author | Kumar, Suneel | en_US |
dc.contributor.author | Akil, Huda | en_US |
dc.contributor.author | Watson, Stanley J. | en_US |
dc.date.accessioned | 2006-04-19T13:35:32Z | |
dc.date.available | 2006-04-19T13:35:32Z | |
dc.date.issued | 2002-03-19 | en_US |
dc.identifier.citation | Norton, Camille S.; Neal, Charles R.; Kumar, Suneel; Akil, Huda; Watson, Stanley J. (2002)."Nociceptin/orphanin FQ and opioid receptor-like receptor mRNA expression in dopamine systems." The Journal of Comparative Neurology 444(4): 358-368. <http://hdl.handle.net/2027.42/34463> | en_US |
dc.identifier.issn | 0021-9967 | en_US |
dc.identifier.issn | 1096-9861 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34463 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11891648&dopt=citation | en_US |
dc.description.abstract | Although nociceptin/orphanin FQ (N/OFQ) influences dopamine (DA) neuronal activity, it is not known whether N/OFQ acts directly on DA neurons, indirectly by means of local circuitry, or both. We used two parallel approaches, dual in situ hybridization (ISH) and neurotoxic lesions of DA neurons by using 6-hydroxydopamine (6-OHDA), to ascertain whether N/OFQ and the N/OFQ receptor (NOP) mRNA are expressed in DA neurons in the ventral tegmental area (VTA) and substantia nigra compacta (SNc). In the VTA and SNc, small populations (∼6–10%) of N/OFQ-containing neurons coexpressed mRNA for tyrosine hydroxylase (TH), the rate-limiting enzyme for DA synthesis. Similarly, very few (1–2%) TH-positive neurons contained N/OFQ mRNA signal. A majority of NOP-positive neurons (∼75%) expressed TH mRNA and roughly half of the TH-containing neurons expressed NOP mRNA. Many N/OFQ neurons (∼50–60%) expressed glutamic acid decarboxylase 65 and 67 mRNAs, markers for Γ-aminobutyric acid (GABA) neurons. In the 6-OHDA lesion studies, NOP mRNA levels were nearly 80 and 85% lower in the VTA and SNc, respectively, on the lesioned side. These lesions appear to lead to compensatory changes, with N/OFQ mRNA levels approximately 60% and 300% higher in the VTA and SNc, respectively, after 6-OHDA lesions. Finally, N/OFQ-stimulated [ 35 S]guanylyl-5′-O-(Γ-thio)-triphosphate levels were decreased in the VTA and SNc but not the prefrontal cortex after 6-OHDA lesions. Accordingly, it appears that N/OFQ mRNA was found largely on nondopaminergic (i.e., GABA) neurons, whereas NOP mRNA was located on DA neurons. N/OFQ is in a position to influence DA neuronal activity by means of the NOP located on DA neurons. J. Comp. Neurol. 444:358–368, 2002. © 2002 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1946957 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Nociceptin/orphanin FQ and opioid receptor-like receptor mRNA expression in dopamine systems | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 ; University of Michigan, Mental Health Research Institute, 205 Zina Pitcher Place, Ann Arbor, MI 48109-0720 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 ; Department of Pediatrics, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 ; Department of Psychiatry, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 ; Department of Psychiatry, University of Michigan Medical Center, Ann Arbor, Michigan 48109-0720 | en_US |
dc.identifier.pmid | 11891648 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34463/1/10154_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/cne.10154 | en_US |
dc.identifier.source | The Journal of Comparative Neurology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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