WDR1 colocalizes with ADF and actin in the normal and noise-damaged chick cochlea
dc.contributor.author | Oh, Seung-Ha | en_US |
dc.contributor.author | Adler, Henry J. | en_US |
dc.contributor.author | Raphael, Yehoash | en_US |
dc.contributor.author | Lomax, Margaret I. | en_US |
dc.date.accessioned | 2006-04-19T13:35:35Z | |
dc.date.available | 2006-04-19T13:35:35Z | |
dc.date.issued | 2002-07-08 | en_US |
dc.identifier.citation | Oh, Seung-Ha; Adler, Henry J.; Raphael, Yehoash; Lomax, Margaret I. (2002)."WDR1 colocalizes with ADF and actin in the normal and noise-damaged chick cochlea." The Journal of Comparative Neurology 448(4): 399-409. <http://hdl.handle.net/2027.42/34464> | en_US |
dc.identifier.issn | 0021-9967 | en_US |
dc.identifier.issn | 1096-9861 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34464 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12115702&dopt=citation | en_US |
dc.description.abstract | Auditory hair cells of birds, unlike hair cells in the mammalian organ of Corti, can regenerate following sound-induced loss. We have identified several genes that are upregulated following such an insult. One gene, WDR1 , encodes the vertebrate homologue of actin-interacting protein 1, which interacts with actin depolymerization factor (ADF) to enhance the rate of actin filament cleavage. We examined WDR1 expression in the developing, mature, and noise-damaged chick cochlea by in situ hybridization and immunocytochemistry. In the mature cochlea, WDR1 mRNA was detected in hair cells, homogene cells, and cuboidal cells, all of which contain high levels of F-actin. In the developing inner ear, WDR1 mRNA was detected in homogene cells and cuboidal cells by embryonic day 7, in the undifferentiated sensory epithelium by day 9, and in hair cells at embryonic day 16. We also demonstrated colocalization of WDR1, ADF, and F-actin in all three cell types in the normal and noise-damaged cochlea. Immediately after acoustic overstimulation, WDR1 mRNA was seen in supporting cells. These cells contribute to the structural integrity of the basilar papilla, the maintenance of the ionic barrier at the reticular lamina, and the generation of new hair cells. These results indicate that one of the immediate responses of the supporting cell after noise exposure is to induce WDR1 gene expression and thus to increase the rate of actin filament turnover. These results suggest that WDR1 may play a role either in restoring cytoskeletal integrity in supporting cells or in a cell signaling pathway required for regeneration. J. Comp. Neurol. 448:399–409, 2002. © 2002 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1322440 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | WDR1 colocalizes with ADF and actin in the normal and noise-damaged chick cochlea | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery; The University of Michigan Medical School, Ann Arbor, Michigan 48109-0506 ; Department of Otolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, 110-744 Seoul, Korea ; Dr. Oh is a scholar of the Korean Science and Engineering Foundation. | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery; The University of Michigan Medical School, Ann Arbor, Michigan 48109-0506 ; Department of Otolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, 110-744 Seoul, Korea | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery; The University of Michigan Medical School, Ann Arbor, Michigan 48109-0506 ; Kresge Hearing Research Institute, 9301E MSRBIII Box 0648, Ann Arbor, Michigan 48109-0648 | en_US |
dc.contributor.affiliationother | NIDCD/NIH, Bethesda, Maryland 20892 | en_US |
dc.identifier.pmid | 12115702 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34464/1/10265_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/cne.10265 | en_US |
dc.identifier.source | The Journal of Comparative Neurology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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