Insulin-like growth factor-I prevents apoptosis in neurons after nerve growth factor withdrawal
dc.contributor.author | Russell, James W. | en_US |
dc.contributor.author | Windebank, Anthony J. | en_US |
dc.contributor.author | Schenone, Angelo | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.date.accessioned | 2006-04-19T13:36:10Z | |
dc.date.available | 2006-04-19T13:36:10Z | |
dc.date.issued | 1998-09-15 | en_US |
dc.identifier.citation | Russell, James W.; Windebank, Anthony J.; Schenone, Angelo; Feldman, Eva L. (1998)."Insulin-like growth factor-I prevents apoptosis in neurons after nerve growth factor withdrawal." Journal of Neurobiology 36(4): 455-467. <http://hdl.handle.net/2027.42/34477> | en_US |
dc.identifier.issn | 0022-3034 | en_US |
dc.identifier.issn | 1097-4695 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34477 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9740019&dopt=citation | en_US |
dc.description.abstract | Insulin-like growth factor-I (IGF-I) is emerging as an important growth factor able to modulate the programmed cell death (PCD) pathway mediated by the cysteine-dependent aspartate proteases (caspases); however, little is known about the effect of IGF-I after nerve growth factor (NGF) withdrawal in neurons. To begin to understand the neuronal death-sparing effect of IGF-I under NGF-free conditions, we tested whether embryonic sensory dorsal root ganglion neurons (DRG) were able to survive in defined serum-free medium in the presence of IGF-I. We further studied the role of IGF-I signaling and caspase inhibition after NGF withdrawal. NGF withdrawal produced histological changes of apoptosis including chromatin condensation, shrinkage of the perikaryon and nucleus, retention of the plasma membrane, and deletion of single cells. Both IGF-I and Boc-aspartyl (OMe)-fluoromethylketone (BAF), a caspase inhibitor, equally reduced apoptosis after NGF withdrawal. The antiapoptotic effect of IGF-I was completely blocked by LY294002, an inhibitor of PI 3-kinase signaling, but not by the mitogen-activated protein (MAP) kinase/extracellular signal-regulated protein kinase (ERK) activated protein kinase inhibitor PD98059. Functional IGF-I receptors were extensively expressed both in rat and human DRG neurons, although they were most abundant in the neuronal growth cone. Collectively, these findings indicate that IGF-I, signaling though the PI-3 kinase pathway, is important in modulating PCD in cultured DRG neurons after NGF withdrawal, and IGF-I may be important in DRG embryogenesis. © 1998 John Wiley & Sons, Inc. J Neurobiol 36: 455–467, 1998 | en_US |
dc.format.extent | 285636 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Insulin-like growth factor-I prevents apoptosis in neurons after nerve growth factor withdrawal | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 200 Zina Pitcher Place, 4414 Kresge III, Box 0588, Ann Arbor, Michigan 48109 ; Veterans Administration Medical Center, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 200 Zina Pitcher Place, 4414 Kresge III, Box 0588, Ann Arbor, Michigan 48109 ; Department of Neurology, University of Michigan, 200 Zina Pitcher Place, 4414 Kresge III, Box 0588, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationother | Molecular Neuroscience Program, Mayo Clinic and Foundation, Rochester, Minnesota 55905 | en_US |
dc.contributor.affiliationother | Department of Neurological Sciences and Rehabilitation, University of Genoa, Genoa, 16132 Italy | en_US |
dc.identifier.pmid | 9740019 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34477/1/1_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-4695(19980915)36:4<455::AID-NEU1>3.0.CO;2-V | en_US |
dc.identifier.source | Journal of Neurobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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