Insulin-like growth factor-I prevents caspase-mediated apoptosis in Schwann cells
dc.contributor.author | Delaney, Catherine L. | en_US |
dc.contributor.author | Cheng, Hsin-Lin | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.date.accessioned | 2006-04-19T13:36:21Z | |
dc.date.available | 2006-04-19T13:36:21Z | |
dc.date.issued | 1999-12 | en_US |
dc.identifier.citation | Delaney, Catherine L.; Cheng, Hsin-Lin; Feldman, Eva L. (1999)."Insulin-like growth factor-I prevents caspase-mediated apoptosis in Schwann cells." Journal of Neurobiology 41(4): 540-548. <http://hdl.handle.net/2027.42/34481> | en_US |
dc.identifier.issn | 0022-3034 | en_US |
dc.identifier.issn | 1097-4695 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34481 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10590177&dopt=citation | en_US |
dc.description.abstract | Both neurons and glia succumb to programmed cell death (PCD) when deprived of growth factors at critical periods in development or following injury. Insulin-like growth factor-I (IGF-I) prevents apoptosis in neurons in vitro. To investigate whether IGF-I can protect Schwann cells (SC) from apoptosis, SC were harvested from postnatal day 3 rats and maintained in serum-containing media until confluency. When cells were switched to serum-free defined media (DM) for 12–72 h, they underwent PCD. Addition of insulin or IGF-I prevented apoptosis. Bisbenzamide staining revealed nuclear condensation and formation of apoptotic bodies in SC grown in DM alone, but SC grown in DM plus IGF-I had normal nuclear morphology. The phosphatidylinositol 3-kinase (PI 3-K) inhibitor LY294002 blocked IGF-I–mediated protection. Caspase-3 activity was rapidly activated upon serum withdrawal in SC, and the caspase inhibitor BAF blocked apoptosis. These results suggest that IGF-I rescues SC from apoptosis via PI 3-K signaling which is upstream from caspase activation. © 1999 John Wiley & Sons, Inc. J Neurobiol 41: 540–548, 1999 | en_US |
dc.format.extent | 103707 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Insulin-like growth factor-I prevents caspase-mediated apoptosis in Schwann cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 200 Zina Pitcher Place, 4414 Kresge III, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 200 Zina Pitcher Place, 4414 Kresge III, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 200 Zina Pitcher Place, 4414 Kresge III, Ann Arbor, Michigan 48109 ; Department of Neurology, University of Michigan, 200 Zina Pitcher Place, 4414 Kresge III, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 10590177 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34481/1/9_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-4695(199912)41:4<540::AID-NEU9>3.0.CO;2-P | en_US |
dc.identifier.source | Journal of Neurobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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