Stability of peptide-condensed plasmid DNA formulations
dc.contributor.author | Adami, Roger C. | en_US |
dc.contributor.author | Collard, Wendy T. | en_US |
dc.contributor.author | Gupta, Shamita A. | en_US |
dc.contributor.author | Kwok, Kai Y. | en_US |
dc.contributor.author | Bonadio, Jeffrey F. | en_US |
dc.contributor.author | Rice, Kevin G. | en_US |
dc.date.accessioned | 2006-04-19T13:36:54Z | |
dc.date.available | 2006-04-19T13:36:54Z | |
dc.date.issued | 1998-06 | en_US |
dc.identifier.citation | Adami, Roger C.; Collard, Wendy T.; Gupta, Shamita A.; Kwok, Kai Y.; Bonadio, Jeffrey; Rice, Kevin G. (1998)."Stability of peptide-condensed plasmid DNA formulations." Journal of Pharmaceutical Sciences 87(6): 678-683. <http://hdl.handle.net/2027.42/34493> | en_US |
dc.identifier.issn | 0022-3549 | en_US |
dc.identifier.issn | 1520-6017 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34493 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9607943&dopt=citation | en_US |
dc.description.abstract | Low molecular weight homogeneous peptides were used to form peptide/DNA condensates. A peptide possessing 18 lysines was found to protect plasmid DNA from serum endonuclease and sonicative-induced degradation whereas a shorter peptide possessing 8 lysines dissociated in 0.1 M sodium chloride and failed to protect DNA from enzymatic degradation. Peptide-condensed DNA showed no change in the ratio of supercoiled to circular DNA following 100 W sonication for up to 60 s and was able to transfect HepG2 cells with equivalent efficiency as untreated condensed plasmid DNA. Alternatively, uncondensed plasmid DNA was rapidly fragmented by sonication and serum endonucleases and resulted in negligible gene expression following condensation with peptide. Cationic lipid/DNA complexes were only partially effective at stabilizing DNA in serum compared to the complete stabilization afforded by peptide/DNA condensation. These results indicate that the stabilization afforded by condensation with a peptide protects DNA during formulation and preserves its structure in serum. These functions are important to achieve optimal gene expression from a nonviral gene delivery system. | en_US |
dc.format.extent | 125344 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry | en_US |
dc.title | Stability of peptide-condensed plasmid DNA formulations | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Divisions of Pharmaceutics and Medicinal Chemistry, College of Pharmacy, University of Michigan Medical School, Ann Arbor, Michigan 48109-1065. | en_US |
dc.contributor.affiliationum | Divisions of Pharmaceutics and Medicinal Chemistry, College of Pharmacy, University of Michigan Medical School, Ann Arbor, Michigan 48109-1065. | en_US |
dc.contributor.affiliationum | Divisions of Pharmaceutics and Medicinal Chemistry, College of Pharmacy, University of Michigan Medical School, Ann Arbor, Michigan 48109-1065. | en_US |
dc.contributor.affiliationum | Divisions of Pharmaceutics and Medicinal Chemistry, College of Pharmacy, University of Michigan Medical School, Ann Arbor, Michigan 48109-1065. | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109-1065. | en_US |
dc.contributor.affiliationum | Divisions of Pharmaceutics and Medicinal Chemistry, College of Pharmacy, University of Michigan Medical School, Ann Arbor, Michigan 48109-1065. ; Divisions of Pharmaceutics and Medicinal Chemistry, College of Pharmacy, University of Michigan Medical School, Ann Arbor, Michigan 48109-1065. | en_US |
dc.identifier.pmid | 9607943 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34493/1/2_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(ISSN)1520-6017 | en_US |
dc.identifier.source | Journal of Pharmaceutical Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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