Metabolic stability of glutaraldehyde cross-linked peptide dna condensates
dc.contributor.author | Adami, Roger C. | en_US |
dc.contributor.author | Rice, Kevin G. | en_US |
dc.date.accessioned | 2006-04-19T13:37:08Z | |
dc.date.available | 2006-04-19T13:37:08Z | |
dc.date.issued | 1999-08 | en_US |
dc.identifier.citation | Adami, Roger C.; Rice, Kevin G. (1999)."Metabolic stability of glutaraldehyde cross-linked peptide dna condensates." Journal of Pharmaceutical Sciences 88(8): 739-746. <http://hdl.handle.net/2027.42/34498> | en_US |
dc.identifier.issn | 0022-3549 | en_US |
dc.identifier.issn | 1520-6017 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34498 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10430535&dopt=citation | en_US |
dc.description.abstract | The stability of peptide DNA condensates was examined after introducing glutaraldehyde to cross-link surface amine groups. A 20 amino acid peptide (CWK 18 ) was used to condense DNA into small (70 nm) condensates. The reaction between glutaraldehyde and peptide DNA condensates was indirectly monitored using a fluorescence-based assay to establish reaction completion in 4–5 h when using glutaraldehyde-to-peptide ratios of 1 to 4 mol equiv. Higher levels of glutaraldehyde cross-linking led to significant increases in particle size. The improved stability imparted by glutaraldehyde cross-linking was demonstrated by the increased resistance of DNA condensates to shear stress induced fragmentation. The cross-linked condensates were also significantly more resistant to in vitro metabolism by serum endonucleases. A decrease in the magnitude of transient gene expression was determined for cross-linked DNA condensates which also resulted in a 10-day steady-state expression when cross-linking with 4 mol equiv of glutaraldehyde. The results suggest that cross-linking DNA condensates may provide a means to alter the time course of transient gene expression by inhibiting DNA metabolism. | en_US |
dc.format.extent | 214290 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry | en_US |
dc.title | Metabolic stability of glutaraldehyde cross-linked peptide dna condensates | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Pharmaceutics, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065. | en_US |
dc.contributor.affiliationum | Division of Pharmaceutics, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065. ; Division of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065. ; Division of Pharmaceutics, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065. | en_US |
dc.identifier.pmid | 10430535 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34498/1/1_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(ISSN)1520-6017 | en_US |
dc.identifier.source | Journal of Pharmaceutical Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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