Bone metastatic LNCaP-derivative C4-2B prostate cancer cell line mineralizes in vitro
dc.contributor.author | Lin, Din-Lii | en_US |
dc.contributor.author | Tarnowski, Catherine P. | en_US |
dc.contributor.author | Zhang, Jian | en_US |
dc.contributor.author | Dai, Jinlu | en_US |
dc.contributor.author | Rohn, Emileigh | en_US |
dc.contributor.author | Patel, Avni H. | en_US |
dc.contributor.author | Morris, Michael D. | en_US |
dc.contributor.author | Keller, Evan T. | en_US |
dc.date.accessioned | 2006-04-19T13:49:05Z | |
dc.date.available | 2006-04-19T13:49:05Z | |
dc.date.issued | 2001-05-15 | en_US |
dc.identifier.citation | Lin, Din-Lii; Tarnowski, Catherine P.; Zhang, Jian; Dai, Jinlu; Rohn, Emileigh; Patel, Avni H.; Morris, Michael D.; Keller, Evan T. (2001)."Bone metastatic LNCaP-derivative C4-2B prostate cancer cell line mineralizes in vitro." The Prostate 47(3): 212-221. <http://hdl.handle.net/2027.42/34759> | en_US |
dc.identifier.issn | 0270-4137 | en_US |
dc.identifier.issn | 1097-0045 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34759 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11351351&dopt=citation | en_US |
dc.description.abstract | BACKGROUND Prostate cancer frequently metastasizes to bone. However, unlike many other tumors that produce osteolytic lesions, prostate cancer produces osteoblastic lesions through unknown mechanisms. In the current study, we explored the ability and mechanism of an osteotropic prostate cancer cell line (C4-2B) to induce mineralization. METHODS C4-2B cells were grown in promineralization media. Mineral deposition was characterized using von Kossa staining, calcium retention, alizarin red staining, Raman spectroscopy, and electron microscopy. Expression of osteoblast-related proteins was determined by RT-PCR. The nuclear level of the bone-specific transcription factor Cbfa1 was determined using western analysis and the effect of inhibiting Cbfa1 function, using a “decoy” Cbfa1 response element oligo, on mineralization was determined. RESULTS The studies demonstrated that C4-2B cells, but not its nonosteotropic parent cell line LNCaP, has an osteoblastlike phenotype including production of alkaline phosphatase, osteocalcin, osteonectin, bone sialoprotein, osteoprotegerin (OPG), and OPG ligand. Most importantly, the C4-2B cells produced hydroxyapatite mineral in vitro. Furthermore, C4-2B cells expressed high nuclear levels of the bone-specific transcription factor Cbfa1, compared to LNCaP cells, which accounts for their ability to produce bone-specific proteins. Inhibition of Cbfa1, using decoy DNA Cbfa1 response elements, abrogated the ability of C4-2B to produce mineral. Finally, we determined that C4-2B cells express bone morphogenic protein-7, a known inducer of Cbfa1 expression. CONCLUSIONS These data demonstrate a novel mechanism through which prostate cancer cells may directly contribute to the osteoblastic component that characterize their skeletal metastatic lesions. Prostate 47:212–221, 2001. © 2001 Wiley-Liss, Inc. | en_US |
dc.format.extent | 564638 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | Bone metastatic LNCaP-derivative C4-2B prostate cancer cell line mineralizes in vitro | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Pathology, School of Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan ; Department of Pathology, School of Medicine, University of Michigan, Ann Arbor, Michigan ; Institute of Gerontology, University of Michigan, Ann Arbor, Michigan ; University of Michigan, Department of Pathology, Unit for Laboratory Animal Medicine, 5305 CCGC, 1500 East Medical Center Drive, Ann Arbor, MI 48109-0940. | en_US |
dc.identifier.pmid | 11351351 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34759/1/1065_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/pros.1065 | en_US |
dc.identifier.source | The Prostate | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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