Gene therapy for bone formation: In vitro and in vivo osteogenic activity of an adenovirus expressing BMP7
dc.contributor.author | Franceschi, Renny T. | en_US |
dc.contributor.author | Wang, Dian | en_US |
dc.contributor.author | Krebsbach, Paul H. | en_US |
dc.contributor.author | Rutherford, R. Bruce | en_US |
dc.date.accessioned | 2006-04-19T13:57:18Z | |
dc.date.available | 2006-04-19T13:57:18Z | |
dc.date.issued | 2000-09-01 | en_US |
dc.identifier.citation | Franceschi, Renny T.; Wang, Dian; Krebsbach, Paul H.; Rutherford, R. Bruce (2000)."Gene therapy for bone formation: In vitro and in vivo osteogenic activity of an adenovirus expressing BMP7." Journal of Cellular Biochemistry 78(3): 476-486. <http://hdl.handle.net/2027.42/34900> | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.issn | 1097-4644 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34900 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10861845&dopt=citation | en_US |
dc.description.abstract | Bone morphogenetic proteins (BMPs) are well-established agents for inducing orthotopic and ectopic bone formation. However, their clinical usefulness as regenerative agents may be limited by a short in vivo half-life and low specific activity. BMP gene therapy is an alternative route for exploiting the bone-inductive activity of this class of molecules. To test the feasibility of this approach, we examined the osteogenic activity of AdCMV-BMP7, an adenovirus containing BMP7 cDNA under control of the CMV promoter that was constructed using Cre/ lox recombination (Hardy et al. [1997] J. Virol. 71:1842–1849). Adenovirus vectors were shown to readily infect a wide variety of cell types in vitro including osteoblasts, fibroblasts, and myoblasts. COS7 cells transduced with AdCMV-BMP7 produced high levels of BMP-7 (approximately 0.5 Μg/10 6 cells). Furthermore, transduction of C2C12 murine myoblast cells with AdCMVBMP-7 suppressed the muscle phenotype and induced in vitro osteoblast differentiation. To test its in vivo biological activity, AdCMV-BMP7 was mixed with a bovine bone-derived collagen carrier (10 8 plaque-forming units virus/site) and was implanted into mouse muscle and dermal pouches. In both cases, an ossicle containing cortical and trabecular bone and a clearly defined marrow cavity formed at the site of virus implantation within 4 weeks. These data demonstrate that AdCMV-BMP7 transduced cells produce biologically active BMP-7 both in vitro and in vivo and show that gene therapy by direct viral transduction using a virus/matrix implant may be a viable route for stimulating bone regeneration. J. Cell. Biochem. 78:476–486, 2000. © 2000 Wiley-Liss, Inc. | en_US |
dc.format.extent | 734977 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Gene therapy for bone formation: In vitro and in vivo osteogenic activity of an adenovirus expressing BMP7 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078 ; Biological Chemistry, School of Medicine, University of Michigan, Ann Arbor, MI 48109-0606 ; Department of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, 1011 North University Avenue, Ann Arbor, MI 48109-1078 | en_US |
dc.contributor.affiliationum | Departments of Periodontics/Prevention/Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078 | en_US |
dc.contributor.affiliationum | Oral Medicine/Pathology/Oncology, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078 | en_US |
dc.contributor.affiliationum | Cariology/Endodontics/Restorative Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078 | en_US |
dc.identifier.pmid | 10861845 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34900/1/12_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/1097-4644(20000901)78:3<476::AID-JCB12>3.0.CO;2-5 | en_US |
dc.identifier.source | Journal of Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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