In vivo measures of dopaminergic radioligands in the rat brain: Equilibrium infusion studies
dc.contributor.author | Kilbourn, Michael R. | en_US |
dc.contributor.author | Sherman, Phillip S. | en_US |
dc.contributor.author | Kuszpit, Kyle | en_US |
dc.date.accessioned | 2006-04-19T14:02:59Z | |
dc.date.available | 2006-04-19T14:02:59Z | |
dc.date.issued | 2002-03-01 | en_US |
dc.identifier.citation | Kilbourn, Michael R.; Sherman, Phillip S.; Kuszpit, Kyle (2002)."In vivo measures of dopaminergic radioligands in the rat brain: Equilibrium infusion studies." Synapse 43(3): 188-194. <http://hdl.handle.net/2027.42/34994> | en_US |
dc.identifier.issn | 0887-4476 | en_US |
dc.identifier.issn | 1098-2396 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34994 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11793424&dopt=citation | en_US |
dc.description.abstract | The application of an equilibrium infusion method for measuring specific in vivo radioligand binding in the conscious rat brain was evaluated for two ligands of the dopaminergic system, (+)-Α-[ 11 C]dihydrotetrabenazine (DTBZ) and d-threo -[ 11 C]methylphenidate (MePhen). Both radioligands can be successfully utilized to reach equilibrium distributions in rat brain within 1 h; combinations of tritiated and carbon-11-labeled radiotracers can furthermore be used to obtain simultaneous measures of the neuronal membrane dopamine transporter (using [ 3 H]MePhen) and vesicular monoamine transporter (using [ 11 C]DTBZ) in the same animal. These studies provided quantitative measures of distribution volume ratios, which represent specific radioligand binding. Stereospecificity of in vivo binding was demonstrated using equilibrium infusions of the low-affinity isomers of each ligand, (−)-Α-[ 11 C]dihydrotetrabenazine (DTBZ) and l-threo -[ 11 C]methylphenidate, both of which produced uniform brain distributions and no specific binding. Specific binding of (+)-Α-[ 11 C]dihydrotetrabenazine was blocked by co-infusion of tetrabenazine, but was unaffected by administration of methylphenidate, haloperidol, or apomorphine. Specific binding of d-threo -[ 11 C]methylphenidate, conversely, was blocked with unlabeled methylphenidate but not affected by tetrabenazine or the dopamine receptor ligands. Equilibrium measures of in vivo radioligand binding, as utilized in this study, offer a quantitative means to evaluate acute and chronic drug effects on in vivo radioligand binding in the rat brain. Synapse 43:188–194, 2002. © 2002 Wiley-Liss, Inc. | en_US |
dc.format.extent | 77349 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | In vivo measures of dopaminergic radioligands in the rat brain: Equilibrium infusion studies | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Division of Nuclear Medicine, 3480 Kresge III Bldg., University of Michigan, Ann Arbor, MI 48109 | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 11793424 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34994/1/10039_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/syn.10039 | en_US |
dc.identifier.source | Synapse | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.