Gamma-irradiation induces matrix metalloproteinase II expression in a p53-dependent manner
dc.contributor.author | Wang, Ju-Lin | en_US |
dc.contributor.author | Sun, Yi | en_US |
dc.contributor.author | Wu, Shiyong | en_US |
dc.date.accessioned | 2006-04-19T14:07:11Z | |
dc.date.available | 2006-04-19T14:07:11Z | |
dc.date.issued | 2000-04 | en_US |
dc.identifier.citation | Wang, Ju-Lin; Sun, Yi; Wu, Shiyong (2000)."Gamma-irradiation induces matrix metalloproteinase II expression in a p53-dependent manner." Molecular Carcinogenesis 27(4): 252-258. <http://hdl.handle.net/2027.42/35060> | en_US |
dc.identifier.issn | 0899-1987 | en_US |
dc.identifier.issn | 1098-2744 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/35060 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10747288&dopt=citation | en_US |
dc.description.abstract | Matrix metalloproteinases (MMPs) are a family of proteinases that degrade the basement membrane and have been implicated in promoting tumor metastasis. MMP-2, one member of this family, was recently found to be a p53 target and subject to p53 upregulation. In this study, we examined the correlation between the expression of MMP-2 and the increased expression of p53 after γ-irradiation. Three human p53-positive cell lines that express wild-type p53, including U2-OS (osteosarcoma), RKO (colon carcinoma), MCF-7 (breast carcinoma), one mouse p53 positive cell line and HepG2 (liver carcinoma), and two p53-negative human cell lines, SAOS-2 (osteosarcoma) and RKO-E6 (colon carcinoma), were used in this study. The MMP-2 activity was analyzed by using gelatin zymography. The p53 level was measured by western blot analysis. Our results show that wild-type p53 induced by ionizing radiation caused a subsequent increase of MMP-2 activity in U2-OS and RKO cells but not in MCF-7, HepG2, SAOS-2, or RKO-E6 cells. These results suggest that the γ-radiation–induced expression of MMP-2 is dependent on the cell type and presence of functional p53. Thus, ionizing radiation could activate MMP-2 activity in a subset of human cancer cells and may lead to an increase in their metastatic potential. Mol. Carcinog. 27:252–258, 2000. © 2000 Wiley-Liss, Inc. | en_US |
dc.format.extent | 173532 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | Gamma-irradiation induces matrix metalloproteinase II expression in a p53-dependent manner | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Radiation Oncology, Division of Radiation and Cancer Biology, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Radiation Oncology, Division of Radiation and Cancer Biology, University of Michigan Medical School, Ann Arbor, Michigan ; Department of Radiation Oncology, Division of Radiation and Cancer Biology, University of Michigan Medical School, Ann Arbor, MI 48109. | en_US |
dc.contributor.affiliationother | Department of Molecular Biology, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan | en_US |
dc.identifier.pmid | 10747288 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/35060/1/2_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1098-2744(200004)27:4<252::AID-MC2>3.0.CO;2-3 | en_US |
dc.identifier.source | Molecular Carcinogenesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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