Detection of Oligonucleotides by External Injection into an Ion Trap Storage/Reflectron Time-of-flight Device
dc.contributor.author | He, Ling | en_US |
dc.contributor.author | Liu, Yan-Hui | en_US |
dc.contributor.author | Zhu, Yongdong | en_US |
dc.contributor.author | Lubman, David M. | en_US |
dc.date.accessioned | 2006-04-19T14:07:31Z | |
dc.date.available | 2006-04-19T14:07:31Z | |
dc.date.issued | 1997-08-30 | en_US |
dc.identifier.citation | He, Ling; Liu, Yan-Hui; Zhu, Yongdong; Lubman, David M. (1997)."Detection of Oligonucleotides by External Injection into an Ion Trap Storage/Reflectron Time-of-flight Device." Rapid Communications in Mass Spectrometry 11(13): 1440-1448. <http://hdl.handle.net/2027.42/35067> | en_US |
dc.identifier.issn | 0951-4198 | en_US |
dc.identifier.issn | 1097-0231 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/35067 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9299762&dopt=citation | en_US |
dc.description.abstract | The detection of oligonucleotides has been studied using external injection of matrix-assisted laser desorption/ionization (MALDI) produced ions into a quadrupole ion trap storage device. The ions stored in the trap are then detected by pulsed DC ejection into a reflectron time-of-flight mass spectrometer. It is shown that by using a low acceleration voltage from the probe tip and external injection into the trap that 3-mers to 9-mers can be stored for 20–50 ms and detected as intact ions. In comparison, direct MALDI inside the trap resulted in extensive fragmentation as a function of storage time. Using the external injection method with a He buffer gas in the trap yields a resolution of 250–400 in the ion trap storage/reflectron time-of-flight mass spectrometer. The resolution can be further enhanced to 400–800 using a bipolar extraction method. In addition, the trapping efficiency for the injection of externally produced ions into the trap can be enhanced using a dynamic trapping method. The MALDI mass spectra produced by this methodology result in excellent signal-to-noise ratio due to the capabilities of the trap for eliminating low mass matrix background from the spectra. © 1997 John Wiley & Sons, Ltd. | en_US |
dc.format.extent | 225602 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Analytical Chemistry and Spectroscopy | en_US |
dc.title | Detection of Oligonucleotides by External Injection into an Ion Trap Storage/Reflectron Time-of-flight Device | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA ; Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.identifier.pmid | 9299762 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/35067/1/25_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-0231(19970830)11:13<1440::AID-RCM25>3.0.CO;2-9 | en_US |
dc.identifier.source | Rapid Communications in Mass Spectrometry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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