The Use of On-line Capillary Electrophoresis/Electrospray Ionization with Detection via an Ion Trap Storage/Reflectron Time-of-flight Mass Spectrometer for Rapid Mutation-site Analysis of Hemoglobin Variants
dc.contributor.author | Li, Michael X. | en_US |
dc.contributor.author | Wu, Jing-Tao | en_US |
dc.contributor.author | Liu, Lin | en_US |
dc.contributor.author | Lubman, David M. | en_US |
dc.date.accessioned | 2006-04-19T14:07:42Z | |
dc.date.available | 2006-04-19T14:07:42Z | |
dc.date.issued | 1997-01-15 | en_US |
dc.identifier.citation | Li, Michael X.; Wu, Jing-Tao; Liu, Lin; Lubman, David M. (1997)."The Use of On-line Capillary Electrophoresis/Electrospray Ionization with Detection via an Ion Trap Storage/Reflectron Time-of-flight Mass Spectrometer for Rapid Mutation-site Analysis of Hemoglobin Variants." Rapid Communications in Mass Spectrometry 11(1): 99-108. <http://hdl.handle.net/2027.42/35071> | en_US |
dc.identifier.issn | 0951-4198 | en_US |
dc.identifier.issn | 1097-0231 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/35071 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9050263&dopt=citation | en_US |
dc.description.abstract | Capillary electrophoresis/electrospray ionization using an ion trap storage/reflectron time-of-flight mass spectrometer detector (CE/ESI-IT/reTOF) is used to provide a rapid and sensitive method for analyzing structural variants in the hemoglobin (Hb) β-chain. The Hb α- and β-chains are separated and the β-chain is digested by trypsin. The digest is analyzed by CE/ESI-IT/reTOF where a comparison of the total ion electrophorograms and mass spectra of the mutant and normal hemoglobins (Hbs) can detect the presence of a mutation site. In addition, collision-induced dissociation in the vacuum interface — skimmer region can be used to pinpoint the identity of such a site. The unique capability of the CE/ESI-IT/reTOF system for accurately detecting fast separations with narrow peaks that may be under 1 s full width at half maximum is demonstrated. The speed of this system is essential for resolution of the large number of peaks that are separated in a short time duration using CE separations. © 1997 John Wiley & Sons, Ltd. | en_US |
dc.format.extent | 210793 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Analytical Chemistry and Spectroscopy | en_US |
dc.title | The Use of On-line Capillary Electrophoresis/Electrospray Ionization with Detection via an Ion Trap Storage/Reflectron Time-of-flight Mass Spectrometer for Rapid Mutation-site Analysis of Hemoglobin Variants | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA ; Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.identifier.pmid | 9050263 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/35071/1/813_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-0231(19970115)11:1<99::AID-RCM813>3.0.CO;2-6 | en_US |
dc.identifier.source | Rapid Communications in Mass Spectrometry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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