Rapid screening of protein profiles of human breast cancer cell lines using non-porous reversed-phase high performance liquid chromatography separation with matrix-assisted laser desorption/ionization time-of-flight mass spectral analysis
dc.contributor.author | Chong, Bathsheba E. | en_US |
dc.contributor.author | Lubman, David M. | en_US |
dc.contributor.author | Miller, Fred R. | en_US |
dc.contributor.author | Rosenspire, Allen J. | en_US |
dc.date.accessioned | 2006-04-19T14:07:59Z | |
dc.date.available | 2006-04-19T14:07:59Z | |
dc.date.issued | 1999-09-30 | en_US |
dc.identifier.citation | Chong, Bathsheba E.; Lubman, David M.; Miller, Fred R.; Rosenspire, Allen J. (1999)."Rapid screening of protein profiles of human breast cancer cell lines using non-porous reversed-phase high performance liquid chromatography separation with matrix-assisted laser desorption/ionization time-of-flight mass spectral analysis." Rapid Communications in Mass Spectrometry 13(18): 1808-1812. <http://hdl.handle.net/2027.42/35076> | en_US |
dc.identifier.issn | 0951-4198 | en_US |
dc.identifier.issn | 1097-0231 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/35076 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10482893&dopt=citation | en_US |
dc.description.abstract | Non-porous reversed-phase (NP-RP) HPLC has been used to rapidly generate protein profiles of whole cell lysates of human breast cancer cell lines. The non-porous packing material used was silica coated with C18, which provided rapid separation with high collection efficiency of proteins from cell lysates. This method was used to study the differences in protein profiles among normal cells and fully malignant cells that share a common genetic background. The highly expressed proteins in each cell type were separated and collected in the liquid state where they were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to obtain the molecular weight of the proteins. The protein fractions were then subjected to tryptic digestion and analyzed by pulsed delay extraction (PDE)-MALDI-TOFMS to obtain the peptide maps. The expressed proteins were identified based upon the molecular weight and peptide map using database-searching procedures. It is shown that key cancer-related proteins can be detected and identified which may be potentially used as biomarkers for cancer detection. Copyright© 1999 John Wiley & Sons, Ltd. | en_US |
dc.format.extent | 72059 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Analytical Chemistry and Spectroscopy | en_US |
dc.title | Rapid screening of protein profiles of human breast cancer cell lines using non-porous reversed-phase high performance liquid chromatography separation with matrix-assisted laser desorption/ionization time-of-flight mass spectral analysis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA ; Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA | en_US |
dc.contributor.affiliationother | Karmanos Cancer Institute, The Detroit Medical Center, Detroit, MI 48201, USA | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, The Wayne State University, Detroit, MI 48202, USA | en_US |
dc.identifier.pmid | 10482893 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/35076/1/720_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-0231(19990930)13:18<1808::AID-RCM720>3.0.CO;2-U | en_US |
dc.identifier.source | Rapid Communications in Mass Spectrometry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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