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Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells
dc.contributor.authorVelkey, J. Matthewen_US
dc.contributor.authorO'Shea, K. Sueen_US
dc.date.accessioned2006-04-19T14:20:09Z
dc.date.available2006-04-19T14:20:09Z
dc.date.issued2003-09en_US
dc.identifier.citationVelkey, J. Matthew; O'Shea, K. Sue (2003)."Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells." genesis 37(1): 18-24. <http://hdl.handle.net/2027.42/35268>en_US
dc.identifier.issn1526-954Xen_US
dc.identifier.issn1526-968Xen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/35268
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14502573&dopt=citationen_US
dc.description.abstractSummary: We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express small stem-loop RNA transcripts corresponding to Oct4 mRNA. Cells transfected with Oct4 RNAi constructs demonstrated reduced levels of Oct4 mRNA and exhibited characteristics of trophectodermal differentiation. These findings support the critical role of Oct4 in regulating stem cell identity and suggest that future experiments using RNAi in ES cells can elucidate the roles of other genes affecting lineage specification during differentiation. genesis 37:18–24, 2003. © 2003 Wiley-Liss, Inc.en_US
dc.format.extent335297 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherGeneticsen_US
dc.titleOct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan ; Department of Cell and Developmental Biology, 4643 Medical Sciences II, University of Michigan Medical School, Ann Arbor, MI 48109-0616en_US
dc.identifier.pmid14502573en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/35268/1/10218_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/gene.10218en_US
dc.identifier.sourcegenesisen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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