Floxed allele for conditional inactivation of the voltage-gated sodium channel Scn8a (Na v 1.6)
dc.contributor.author | Levin, Stephen I. | en_US |
dc.contributor.author | Meisler, Miriam H. | en_US |
dc.date.accessioned | 2006-04-19T14:20:15Z | |
dc.date.available | 2006-04-19T14:20:15Z | |
dc.date.issued | 2004-08 | en_US |
dc.identifier.citation | Levin, Stephen I.; Meisler, Miriam H. (2004)."Floxed allele for conditional inactivation of the voltage-gated sodium channel Scn8a (Na v 1.6)." genesis 39(4): 234-239. <http://hdl.handle.net/2027.42/35270> | en_US |
dc.identifier.issn | 1526-954X | en_US |
dc.identifier.issn | 1526-968X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/35270 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15286995&dopt=citation | en_US |
dc.description.abstract | The sodium channel gene Scn8a encodes the channel Na V 1.6, which is widely distributed in the central and peripheral nervous system. Na V 1.6 is the major channel at the nodes of Ranvier in myelinated axons. Mutant alleles of mouse Scn8a result in neurological disorders including ataxia, tremor, paralysis, and dystonia. We generated a floxed allele of Scn8a by inserting loxP sites around the first coding exon. The initial targeted allele containing the neo-cassette was a severe hypomorph. In vivo deletion of the neo-cassette by Flp recombinase produced a floxed allele that generates normal expression of Na V 1.6 protein. Ubiquitous deletion of the floxed exon by Cre recombinase in ZP3-Cre transgenic mice produced the Scn8a del allele. The null phenotype of Scn8a del homozygotes confirms the in vivo inactivation of Scn8a . Conditional inactivation of the floxed allele will make it possible to circumvent the lethality that results from complete loss of Scn8a in order to investigate the physiologic role of Na V 1.6 in subpopulations of neurons. genesis 39:234–239, 2004. © 2004 Wiley-Liss, Inc. | en_US |
dc.format.extent | 236186 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Genetics | en_US |
dc.title | Floxed allele for conditional inactivation of the voltage-gated sodium channel Scn8a (Na v 1.6) | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor, Michigan ; Unit for Laboratory Animal Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor, Michigan ; Department of Human Genetics, 4909 Buhl Box 0618, University of Michigan, Ann Arbor, MI 48109-0618 | en_US |
dc.identifier.pmid | 15286995 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/35270/1/20050_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/gene.20050 | en_US |
dc.identifier.source | genesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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