Connective tissue activation
dc.contributor.author | Castor, C. William | en_US |
dc.contributor.author | Ritchie, James C. | en_US |
dc.contributor.author | Williams, Charles H., Jr. | en_US |
dc.contributor.author | Scott, Mary E. | en_US |
dc.contributor.author | Whitney, Sherry L. | en_US |
dc.contributor.author | Myers, Stephen L. | en_US |
dc.contributor.author | Sloan, Tod B. | en_US |
dc.contributor.author | Anderson, Byron E. | en_US |
dc.date.accessioned | 2006-04-28T16:22:06Z | |
dc.date.available | 2006-04-28T16:22:06Z | |
dc.date.issued | 1979-03 | en_US |
dc.identifier.citation | Castor, C. William; Ritchie, James C.; Williams, Charles H.; Scott, Mary E.; Whitney, Sherry L.; Myers, Stephen L.; Sloan, Tod B.; Anderson, Byron E. (1979)."Connective tissue activation." Arthritis & Rheumatism 22(3): 260-272. <http://hdl.handle.net/2027.42/37739> | en_US |
dc.identifier.issn | 0004-3591 | en_US |
dc.identifier.issn | 1529-0131 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37739 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=420716&dopt=citation | en_US |
dc.description.abstract | Connective tissue activating peptide-III (CTAP-III) isolated from human platelets is a potent mitogen for human connective tissue cells in culture in addition to stimulating glycosaminoglycan synthesis, glucose consumption, and lactate formation. The amino acid composition of apparently homogeneous CTAP-III was determined, confirming the presence of two disulfide links and providing a calculated molecular weight of 11,633 daltons. Comparison of the mitogenic activity of serum and plasma-serum suggests that CTAP-III is a major mitogenic component of human serum. Seventeen strains of human connective tissue cells (synovial, cartilage, dermal and thyroid) incorporated [ 3 H]-thymidine at up to 30 times control at levels under the influence of microgram quantities of CTAP-III and caused detectable increases in thymidine incorporation at levels as low as 10–29 ng/ml. Prostaglandin E 1 (0.01 Μg/ml) and dibutyryl cyclic AMP (25 Μg/ml) potentiated the glycosaminoglycan stimulating effect of CTAP-III, but not its mitogenic effect. Cycloheximide and actinomycin D blocked the biologic actions of CTAP-III. Cortisol and penicillamine had little effect on the mitogenic activity of CTAP-III, whereas antirheumatic agents such as acetylsalicylic acid and phenylbutazone opposed the mitogenic activity when added to cultures at clinically relevant concentrations. A weak antiheparin factor secreted by platelets, low affinity platelet factor 4 (LA-PF 4 ), was shown to be similar to CTAP-III in biologic actions, electrophoretic mobility, amino acid composition, and antigenic determinants. | en_US |
dc.format.extent | 1376275 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Rheumatology | en_US |
dc.title | Connective tissue activation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Geriatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. ; Rackham Arthritis Research Unit, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Rackham Arthritis Research Unit, the University of Michigan Medical School, and the Veterans Administration Hospital, Ann Arbor, Michigan, and the Departments of Biochemistry, Otolaryngology and Maxillofacial Surgery, Northwestern University Medical and Dental Schools, Chicago, Illinois. | en_US |
dc.identifier.pmid | 420716 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37739/1/1780220308_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/art.1780220308 | en_US |
dc.identifier.source | Arthritis & Rheumatism | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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