Solution properties and structure of brain proteolipids
dc.contributor.author | Zand, Robert | en_US |
dc.date.accessioned | 2006-04-28T16:26:17Z | |
dc.date.available | 2006-04-28T16:26:17Z | |
dc.date.issued | 1968-07 | en_US |
dc.identifier.citation | Zand, Robert (1968)."Solution properties and structure of brain proteolipids." Biopolymers 6(7): 939-953. <http://hdl.handle.net/2027.42/37822> | en_US |
dc.identifier.issn | 0006-3525 | en_US |
dc.identifier.issn | 1097-0282 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37822 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=5657911&dopt=citation | en_US |
dc.description.abstract | Bovine white matter proteolipids have been studied by several physical methods and have been found to exist as micelles in 2 : 1 (v/v) chloroform–methanol solution. The data would indicate the existence of a critical micelle concentration at 0.017–0.022 g/100 ml. The curve appears linear in the range 0.017–0.2 g/100 ml, but from the data at higher concentrations it would appear that a change in slope is occurring in the region 0.2–0.3 g/100 ml. Light-scattering measurements on 2 : 1 (v/v) chloroform–methanol solutions containing more than 0.2 g/100 ml of proteolipid yielded a weight-average aggregate weight of 2.9 × 10 6 and a radius of gyration of 64.5 Å. The intrinsic viscosity of the solutions was 0.32 dl/g and the Huggins constant was 1.085. Light-scattering measurements in 88.5% formic acid–0.5 M sodium formate yielded a weight-average aggregate weight of 7.1 × 10 6 and a radius of gyration of 241 Å. The intrinsic viscosity observed for this solvent system is 0.14 dl/g and the Huggins constant is 1.005. Osmotic pressure measurements in 2 : 1 (v/v) chloroform–methanol containing less than 0.2 g/ 100 ml of proteolipid yielded a number-average aggregate weight of 7.2 × 10 4 Ultracentrifugal analysis in 1.5:1 (v/v) methylene chloride–methanol showed two broad peaks with, s values of s 1.5% = 20.05 S, s 2% = 19.79 S for the minor peak and s 1.5%,2% = 1.86 S for the major peak. Optical rotatory dispersion studies revealed large changes in b 0 with change in solvent and proteolipid concentration. The present data suggest that the mode of attachment of protein to lipid is primarily of a noncovalent type. The results of this investigation also suggest that the proteolipid micelle above 0.2 g/100 ml is cylindrical (prolate ellipsoid) in 2:1 (v/v) chloroform-methanol and approaches a more spherical shape in 88.5% formic acid. A structure for the proteolipid micellar complex above concentrations of 0.2 g/100 ml is proposed. | en_US |
dc.format.extent | 791209 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Solution properties and structure of brain proteolipids | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Biophysics Research Division, Institute of Science and Technology, The University of Michigan, Ann Arbor, Michigan 48104 | en_US |
dc.identifier.pmid | 5657911 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37822/1/360060705_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/bip.1968.360060705 | en_US |
dc.identifier.source | Biopolymers | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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