Calorimetric studies of the polynucleotide analog poly(−)2-[2-(thymin-1-yl) propanamido]propenoic acid
dc.contributor.author | Zand, Robert | en_US |
dc.contributor.author | Jiang, Z. Z. | en_US |
dc.contributor.author | Overberger, Charles Gilbert | en_US |
dc.date.accessioned | 2006-04-28T16:27:45Z | |
dc.date.available | 2006-04-28T16:27:45Z | |
dc.date.issued | 1986-05 | en_US |
dc.identifier.citation | Zand, R.; Jiang, Z. Z.; Overberger, C. G. (1986)."Calorimetric studies of the polynucleotide analog poly(−)2-[2-(thymin-1-yl) propanamido]propenoic acid." Biopolymers 25(5): 787-794. <http://hdl.handle.net/2027.42/37852> | en_US |
dc.identifier.issn | 0006-3525 | en_US |
dc.identifier.issn | 1097-0282 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37852 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2424520&dopt=citation | en_US |
dc.description.abstract | The thermally elicited structural and conformational transitions of the polynucleotide analog, poly(-)-2-[2-(thymin-1-yl)propanamido]propenoic acid, P(-)TDHA, have been studied using differential scanning calorimetry (DSC), differential thermal analysis (DTA), and thermogravmetric analysis (TGA). The differential scanning calorimetry curves obtained on solid P(-)TDHA samples exhibited five distinct transitions. The transition occurring at 50°C is attributed to a disruption of interactions involving thymine–thymine stacking. In contrast the transition observed at 83°C is attributed to a hydrogen-bonding interaction involving the thymine residues, whereas the transition occurring at 110°C is assigned to hydrogen bonding of the carboxylic acid side-chain groups. The transitions observed at 50, 83, and 110°C are reversible if the heated and quenched sample is allowed to equilibrate in an atmosphere of high humidity. The transition occurring at 127°C is viewed as a structural rearrangement of the polymer backbone that does not involve the participation of water molecules. The transition observed at a temperature above 197°C is attributed to a structural modification of the polymer resulting from decomposition. Solutions of P(-)TDHA in 0.1 M phosphate buffer at pH 7.05 showed only a single transition at 50°C, which is in accord with an observed transition in the solid state assigned to the disruption of base-stacking interactions. The average enthalpy for the transition at 50°C was 0.92 cal/g in the solid state and 1.08 cal/g for the solution, which provides additional support for the assignment. | en_US |
dc.format.extent | 446690 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Calorimetric studies of the polynucleotide analog poly(−)2-[2-(thymin-1-yl) propanamido]propenoic acid | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Macromolecular Research Center and Biophysics Research Division, Institute of Science and Technology, Department of Biological Chemistry and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 ; Macromolecular Research Center and Biophysics Research Division, Institute of Science and Technology, Department of Biological Chemistry and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Macromolecular Research Center and Biophysics Research Division, Institute of Science and Technology, Department of Biological Chemistry and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Macromolecular Research Center and Biophysics Research Division, Institute of Science and Technology, Department of Biological Chemistry and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 2424520 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37852/1/360250504_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/bip.360250504 | en_US |
dc.identifier.source | Biopolymers | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.