Substrate-dependent differences in production of extracellular matrix molecules by squamous carcinoma cells and diploid fibroblasts
dc.contributor.author | Varani, James | en_US |
dc.contributor.author | Fligiel, Suzanne E. G. | en_US |
dc.contributor.author | Inman, Dennis R. | en_US |
dc.contributor.author | Helmreich, David L. | en_US |
dc.contributor.author | Bendelow, Matthew J. | en_US |
dc.contributor.author | Hillegas, William A. | en_US |
dc.date.accessioned | 2006-04-28T16:29:58Z | |
dc.date.available | 2006-04-28T16:29:58Z | |
dc.date.issued | 1989-04-20 | en_US |
dc.identifier.citation | Varani, James; Fligiel, Suzanne E. G.; Inman, Dennis R.; Helmreich, David L.; Bendelow, Matthew J.; Hillegas, William (1989)."Substrate-dependent differences in production of extracellular matrix molecules by squamous carcinoma cells and diploid fibroblasts." Biotechnology and Bioengineering 33(10): 1235-1241. <http://hdl.handle.net/2027.42/37897> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37897 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18587855&dopt=citation | en_US |
dc.description.abstract | Two human squamous carcinoma cell lines and human diploid fibroblasts were examined for the production of extracellular matrix (ECM) molecules including fibronectin (FN), laminin (LN), and thrombospondin (TSP) when grown on a number of different substrates. The substrates used included glass, plastic, collagen (gelatin), and DEAE-dextran. Levels of TSP as indicated by enzyme-linked immunosorbent assay did not vary significantly as a function of substrate. In contrast, LN levels in the culture medium were significantly decreased when the cells were grown on DEAE-dextran or collagen-linked dextran as compared to the other substrates. FN levels were slightly lower in the culture medium of the cells grown on DEAE-dextran. Biosynthetic labeling followed by immunoprecipitation indicated that the reduction in LN was due, in part, to decreased biosynthesis. Previous studies have indicated that LN influences the behavior of epithelial cells in culture and that the cells, themselves, are a major source of the LN. The differences in LN production noted here indicate that the production of this ECM component is influenced by the substratum on which the cells are grown. These differences could contribute to alterations in biological properties that are known to be influenced by the substratum. | en_US |
dc.format.extent | 884142 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Substrate-dependent differences in production of extracellular matrix molecules by squamous carcinoma cells and diploid fibroblasts | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, Solohill Engineering, Inc., Ann Arbor, Michigan 48104, and Department of Pathology, VAMC-Wayne State University, Allen Park, Michigan 48101 ; Department of Pathology, Univerrsity of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, Solohill Engineering, Inc., Ann Arbor, Michigan 48104, and Department of Pathology, VAMC-Wayne State University, Allen Park, Michigan 48101 | en_US |
dc.contributor.affiliationum | Department of pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, Solohill Engineering, Inc., Ann Arbor, Michigan 48104, and Department of Pathology, VAMC-Wayne State University, Allen Park, Michigan 48101 | en_US |
dc.contributor.affiliationum | Department of pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, Solohill Engineering, Inc., Ann Arbor, Michigan 48104, and Department of Pathology, VAMC-Wayne State University, Allen Park, Michigan 48101 | en_US |
dc.contributor.affiliationum | Department of pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, Solohill Engineering, Inc., Ann Arbor, Michigan 48104, and Department of Pathology, VAMC-Wayne State University, Allen Park, Michigan 48101 | en_US |
dc.contributor.affiliationum | Department of pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, Solohill Engineering, Inc., Ann Arbor, Michigan 48104, and Department of Pathology, VAMC-Wayne State University, Allen Park, Michigan 48101 | en_US |
dc.identifier.pmid | 18587855 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37897/1/260331003_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/bit.260331003 | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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