Interaction of infectious viral particles with a quaternary ammonium chlorid (QAC) surface
dc.contributor.author | Tsao, I-Fu | en_US |
dc.contributor.author | Wang, Henry Y. | en_US |
dc.contributor.author | Shipman, Charles Jr. | en_US |
dc.date.accessioned | 2006-04-28T16:30:07Z | |
dc.date.available | 2006-04-28T16:30:07Z | |
dc.date.issued | 1989-08-20 | en_US |
dc.identifier.citation | Tsao, I-Fu; Wang, Henry Y.; Shipman, Charles (1989)."Interaction of infectious viral particles with a quaternary ammonium chlorid (QAC) surface." Biotechnology and Bioengineering 34(5): 639-646. <http://hdl.handle.net/2027.42/37900> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37900 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18588148&dopt=citation | en_US |
dc.description.abstract | The antiviral activity of a surface-bonded quaternary ammonium chloride (QAC) was examined in this study. The mechanism of inactivation was elucidated by a combination of infectivity assay, radioactive labeling assay, and sedimentation analysis. Although the virions are still infectious when attached onto the chemically modified surface, we found these viruses are inactivated if they are eluted from the surface. The inactivation is caused by the disruption of the viral envelope with subsequent release of the nucleocapsid. No evidence indicates the released nucleocapsid is further disrupted. An enveloped virus shows a much higher affinity for the QAC-treated surface than a nonenveloped one due to hydrophobic interaction. The QAC-treated beads can effectively remove the enveloped viruses at low protein concentrations. The titer of herpes simplex virus was reduced by a factor of nearly 5 logarithm units in a 0.5 wt % bovine serum albumin solution with less that 10% protein loss. However, the presence of proteins in the solution reduced both the rate and capacity of this nonspecific adsorption–inactivation process. As a consequence, the removal efficiency is relatively poor in solutions with high protein content. | en_US |
dc.format.extent | 742372 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Interaction of infectious viral particles with a quaternary ammonium chlorid (QAC) surface | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109 ; Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 18588148 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37900/1/260340508_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/bit.260340508 | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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