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A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose

dc.contributor.authorSakoda, Akiyoshien_US
dc.contributor.authorWang, Henry Y.en_US
dc.date.accessioned2006-04-28T16:30:10Z
dc.date.available2006-04-28T16:30:10Z
dc.date.issued1989-10-20en_US
dc.identifier.citationSakoda, Akiyoshi; Wang, Henry Y. (1989)."A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose." Biotechnology and Bioengineering 34(8): 1098-1103. <http://hdl.handle.net/2027.42/37901>en_US
dc.identifier.issn0006-3592en_US
dc.identifier.issn1097-0290en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/37901
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18588204&dopt=citationen_US
dc.description.abstractA new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods.en_US
dc.format.extent615606 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherChemistryen_US
dc.subject.otherBiochemistry and Biotechnologyen_US
dc.titleA new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agaroseen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbsecondlevelMathematicsen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelStatistics and Numeric Dataen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109en_US
dc.identifier.pmid18588204en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/37901/1/260340811_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/bit.260340811en_US
dc.identifier.sourceBiotechnology and Bioengineeringen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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